eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
1
18
10.21608/eajbsa.2018.17728
17728
Toxicity of Some Essential Oils against Myiasis-Producing Fly, Megaselia scalaris, and their Impacts on Proteins and Detoxification Enzymes.
Saleh A.
e_ismail2000@yahoo.com
1
Rabab Abd El-Gawad
2
Biological and Geological Sciences Dept., Fac. of Education, Ain Shams Univ., Egypt
Biological and Geological Sciences Dept., Fac. of Education, Ain Shams Univ., Egypt
Megaselia scalaris(Diptera: Phoridae) is regarded as a medically important insect worldwidebecause it is the source of different myiasis diseases. Therefore, it should becontrolled for human welfare and for economic reasons. Accordingly, the larvicidal activity of six plant essential oils wasdetermined against M. scalaris and their impacts on proteins andisozymes were also estimated in whole body homogenate of the third larvalinstars. The tested essential oils were chamomile (Matricaria chamomilla),clove (Syzygium aromaticum), orange (Citrus sinensis), rosemary (Rosemarinusofficinalis), spearmint (Mentha spicata) and sweet basil (Ocimumbasilicum). Results exhibited that the clove oil had the highest larvicidalactivity, LC50 is 2.38 ml/100g media, whereas, chamomile oil was theleast one, LC50 is 5.37 ml/100g media. Significantconcentration-mortality responses of the treated larvae were observed towardsall tested oils. Concerning protein estimation, the control protein content was12.80±2.68 mg/g tissue, and the oil application produced an elevation in totalprotein contents in treated larvae. This elevation was significant (p<0.05)with clove, orange and spearmint oils amounted to be 92.19, 70.31 and 57.58%,respectively, compared to that of the control larvae, but insignificant (p>0.05)with chamomile, rosemary and sweet basil oils. The electrophoretic studyrevealed that the maximum number of native protein bands was seven atapproximately rate of flow ranging between 0.14 to 0.72 in the control andclove, orange, spearmint and sweet basil treatments. One newly formed proteinband was observed in whole body homogenate of clove, orange, rosemary,spearmint and sweet basil treatments. The profiles of β-esterase,alcohol dehydrogenase and aldehyde oxidase showed the same patterns in bothcontrol and treated larvae. On the other hand, there was a reduction in theisozymes amount percent in the treated larvae as compared with the controlvalues. The biochemical changes in native proteins and isozymes could be usedas a biochemical indicator of toxic stress.
https://eajbsa.journals.ekb.eg/article_17728_4582d46d854a4e9ad69d578753ab3d53.pdf
Megaselia scalaris
Essential oils
toxicity
protein
isozymes
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
19
30
10.21608/eajbsa.2018.17729
17729
Original Article
Insecticidal Activity of Moringa oleifera Synthesized Silver and Zinc Nanoparticles against the House Fly, Musca domestica L.
Rabab Abdel-Gawad
amagdy59@yahoo.com
1
Biological and Geological Sciences Dept., Fac. of Education, Ain Shams Univ., Egypt.
Green synthesizednanoparticles have been studied as newand more effective biopesticides against insects of medical importance and thisfor their eco-friendly aspects. In the current study Moringa oleiferaleaf extract (Mo-LE) was used to synthesis silver nanoparticles (Mo-AgNPs) andzinc oxide nanoparticles (Mo-ZnONPs). Low concentrations of Mo-LE, Mo-AgNPs andMo-ZnONPs showed the larvicidal and pupicidal toxicityagainst Musca domestica. In larvicidal bioassay, the values of LC50were 16.50, 2.03 and 6.41 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,respectively. In addition, the LC50 was determined against pupae,which were129.77, 9.604 and 17.10 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,respectively. Moreover, treatment larvae with LC50's of Mo-LE, Mo-AgNPs andMo-ZnONPsproduced a significant reduction (P<0.05) in the fecundity of femaleand a highly significant reduction (P<0.01) in the egg hatchability.The total protein content and activities of nonspecific esterases, acetylcholine esterase, and glutathione S-transferase enzymes were significantly declined afterlarvae had fed on LC50 Mo-AgNPs andMo-ZnONPstreated diets as compared with the control. This study indicates that it isfeasible to use M. oleifera synthesized nanoparticles as effective candidates to develop newer and cheapcontrol tools for immature stages of M. domestica.
https://eajbsa.journals.ekb.eg/article_17729_8f47e9e38abab9b57dc1fd72a028cd88.pdf
Musca domestica
Moringa oleifera
silver nanoparticles
zinc oxide nanoparticles larvicidal
pupicidal
fecundity
Total protein
detoxification enzymes
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
31
40
10.21608/eajbsa.2018.17730
17730
Original Article
Population Fluctuations and Infestation Rates of the Peach and Mediterranean Fruit Flies on Fig Fruits in Relation to the Prevailing Weather Factors in Fayoum Governorate
Amira Negm
aliamin1969@yahoo.com
1
Plant Protection Research Institute, Agric. Res. Center, Giza, Egypt
The Egyptian fruits are attacked by two of the most harmful tephritid pests, the peach fruit fly, Bactrocera zonata (Saunders), and the Mediterranean fruit fly, Ceratitis capitata (Wiedmann) causing considerable damage. In Fayoum governorate, fig trees are cultivated in condensed plantations to produce their fruits in two overlapped crops per year from July till December. This paper was contributed to saving more information about the occurrence of B. zonata and C. capitata flies in a fig cultivated areas in Fayoum governorate in relation to the effect of various weather factors on their activity and estimating the mentioned fruit flies infestation rates. During the 1st season (2016), the highest peaks of B. zonata and C. capitata population were observed during the 2nd week of October and 1st week of November with respective mean values of captured flies per trap per day (FTD) of 0.48 and 0.74 fly/trap/day. While during the 2nd season (2017), the highest peak of B. zonata and C. capitata males were observed during the 1st week of September and 2nd week of November with a mean FTD values of 1.31 and 0.62 flies/trap/day, respectively. Concerning infestation rates, during the 1st season, the highest mean percentage of fruit infestation was observed during the 1st week of October with a mean of 4.32% occurring by B. zonata females indicating by the emerged flies of B. zonata flies from sampled infested fruits. The fig fruits infestation by B. zonata females continued until the 1st week of November, while, C. capitata females started to attack fig fruits during the 4th week of October and continued until the 3rd week of November. Concerning the 2nd season(2017), the highest mean percentage of fig fruits was recorded during the 1st week of September with a mean of 5.42% occurring by B. zonata females, while, C. capitata infestation on fig fruits was observed firstly during the 2nd week of October and continued until the 2nd week of December coinciding with end of fig fruits harvesting. Throughout the two studied seasons, the trapped males of B. zonata were negatively and insignificantly correlated with both of maximum and minimum temperatures, while, the relative humidity % affected positively insignificantly and positively significantly on B. zonata males activity during 2016 and 2017, respectively. On the other side, C. capitata trapped males were significantly and negatively correlated with maximum and minimum temperatures, while, relative humidity reflected a significant positive correlation with the population of the fruit fly.
https://eajbsa.journals.ekb.eg/article_17730_899d14662d744a1e5e4ad780df436208.pdf
Bactrocera zonata – Ceratitis capitata – fig – Ficus carica- population
ecology – infestation – temperature – relative humidity- Fayoum
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
41
50
10.21608/eajbsa.2018.17731
17731
Original Article
Impact of Biotic and Abiotic Factors on the Population Dynamics of Bemisia tabaci (Genn.) and Tetranychus urticae (Koch) Infested Tomato Plant Lycopersicon esculentum L. at kafr El sheikh Governorate
Afaf Abasse
1
Abd-El wahab H.
2
Naema A.
3
Sawsan A.
4
Mohammed M.
drenayat2017@gmail.com
5
Zoology Department- Faculty of Science (Girls) - Al-Azhar University
Plant Protection Research Institute Dokki- Giza, Egypt
Zoology Department- Faculty of Science (Girls) - Al-Azhar University
Microbiology Department Atomic Energy Authority- Egypt
Zoology Department- Faculty of Science (Girls) - Al-Azhar University
Impact of abiotic (minimum–maximum temperatures andrelative humidity) and biotic factors ( Coccinella undecimpunctata, Syrphus corollae , Amblyseius swirskii, Nesidiocoristenuis and Chrysoperla carnea) on the population dynamicsof Bemisiatabaci (Genn.) and Tetranchys urticae (Koch) infesting tomato plant Lycopersiconesculentum L. at kafr El sheikhgovernorate, Egypt were studied on spring plantation of 2016 &2017 seasons. The analysis ofvariance revealed significant variation among dates of observations, lines andin their interaction for B. tabaci(Genn.), T. urticae (Koch) and their natural enemies. The mean number ofB. tabaci and T. urticaeduring the first season was higher than that was recorded during the secondseason. The percentage of explained variance (E.V.) of abiotic andbiotic altogether in the population densities of B.tabaci and T. urticae in the first season was stronger percentagevalues than second as (62% - 86%) and (94% - 77%) for eggs and nymphsof B.tabaci while (84% _ 77%) and (84% -98%) and for eggs and movable stages of T.urticae during spring of plantation 2016 &2017 seasons, respectively.
https://eajbsa.journals.ekb.eg/article_17731_a29911dcce2e11fe7db5b0efd14490b9.pdf
abiotic & biotic factors
Coccinella undecimpunctata
Syrphus corollae
Amblyseius swirskii
Nesidiocoris tenuis
Chrysoperla carnea
Bemisia tabaci (Genn.) and Tetranychus urticae (Koch)
tomato plant
Lycopersicon esculentum L
Minimum & Maximum temperature and Relative humidity
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
51
58
10.21608/eajbsa.2018.17732
17732
Original Article
Occurrence of Different Mites associated with Different Cereals and Legumes Crops in different locations of Egypt
Yassin A.
essammohamedabdelsalam@gmail.com
1
Osman A.
2
A. Rahouma
3
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
This study proves the occurrence of 60 mite species belonging to 42 genera under 22 families. These mites are belonging to 4 suborders Acaridida (Astigmata), Actinedida (Prostigmata), Gamasida (Mesostigmata) and Oribatida (Cryptostigmata). The astigmatid mites in this study were represented by 7 families, 17 genera and 23 different species. On the other hand the number of mites' species in suborder Prostigmata was 26 species in 18 genera belong to 10 families. The obtained data indicated that the mesostigmatids were represented by 9 different species in 5 genera and 3 different families. The current study showed that the mites that belong to suborder Cryptostigmata were 2 mites only, 2 genera in 2 families. The most abundant mites were Tyrophagus putrescentiae, Caloglyphus berlesi and Rhizoglyphus echinopus (Acaridae); Suidasia nesbitti (Suidasidae); Glycyphagus domesticus (Glycyphagidae); Dermatophagoides farinae (Pyroglyphidae); Cheyletus malaccensis and C. eruditus (Cheyletidae); Pymotes herfesi (Pyemotidae); Cunaxa capreolus (Cunaxidae); Orthotydeus californicus and O. kochi (Tydeidae); Blattisocius keegani and Proctolaelaps pygmaeus (Ascidae). The stigmaeid mite, S. africanus was determined in all tested regions with high numbers.
https://eajbsa.journals.ekb.eg/article_17732_4312bdffa8917a84bf97f65303f329d1.pdf
Bactrocera zonata – Ceratitis capitata – fig – Ficus carica- population
ecology – infestation – temperature – relative humidity- Fayoum
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
59
71
10.21608/eajbsa.2018.17733
17733
Original Article
Venom Composition of Egyptian and Carniolan Honeybee, Apis mellifera L. Affected by Collection Methods.
Hind Zidan
dr.hindzidanamer@gmail.com
1
Zahia Mostafa
2
Mahmoud Ibrahim
3
S. Haggag
4
Doaa Darwish
5
Abir Elfiky
6
Plant Protection Research Institute, Agriculture Research Centre
Entomology Department, Faculty of Science, Ain Shams University
Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Giza, Egypt
Plant Protection Research Institute, Agriculture Research Centre
Molecular Biology Department, National Research Centre, El-Tahrir st., Dokki, Giza, Egypt
Director of ANDI center of excellence in antivenom research, Vacsera, Egypt
Bee venom is one of theeconomic income sources for beekeepers due to their widely medical uses andother traded products. This study investigates the biological activity ofvenom extracts from the honeybee, Apis melliferasubspecies: A. m. lamarckii and A. m. carnica by manual and electro-stimulated extracted methods. Venoms extracted compared with regard to their SDS-PAGE analysis, enzymaticactivities: protease, PLA2, hyaluronidase and haemolyticactivity of melittin, and LD50 value. Theelectrophoretic analysis data showed that manual venoms extract for Egyptianand Carniolan bees contain 24 and 21 bands with molecular weights ranging from127.66 - 15.92 KDa and 133.61- 14.4 KDa, respectively. It was noted that thevenoms electrically extracted for Egyptian and Carniolan bees have the highestsimilarities with 0.363. The enzymatic activities andLD50 value datashowed that the venoms extracted using electrical stimulatedmethod has high optimal quality and it can be commercialized by the beekeeper. The venom of Egyptian subspecies A. m. lamarckii has high biological activityand is the better form for the best pharmacologicalsource. The experimental data can be used for identification, standardizationand determining the biological activity of honeybee venom in Egypt.
https://eajbsa.journals.ekb.eg/article_17733_c26e9ee03a757449a44c51888fd66c6e.pdf
honeybee subspecies/ bee venom/ collection methods / SDS-PAGE / enzyme activities / LD50
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
73
81
10.21608/eajbsa.2018.17734
17734
Original Article
Mites Inhabiting Some Fruit Trees in Ismailia Governorate
Neivin Hussian
1
Hamdy El-Sharabasy
2
Ahmed Aboughalia
ahaboghalia@yahoo.com
3
Maha Soliman
maha_soliman@hotmail.com
4
Plant protection Research Institute, Agricultural Research Center, Dokki, Egypt
Plant protection Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Zoology Department, Faculty of Science, Suez Canal University, Egypt
Zoology Department, Faculty of Science, Suez Canal University, Egypt
Field study was carried out on mite species inhabiting some fruit trees in Ismailia Governorate, during the period from October 2014 to September 2016. Survey of mites proved the occurrence of 44 mite species belonging to 36 genera and 21 families belong to 2 orders and 4 suborders. Order: Acariformes include three suborders; Prostigmata, Astigmata and Cryptostigmata. Suborder Prostigmata was represented by 14 families: Tetranychidae (7 species), Tenuipalpidae (8 species), Eriophyidae (one species), Stigmaeidae (2 species), Neophyllobiidae (2 species), Caligonellidae (one species), Raphignathidae (one species), Cheyletidae (3 species), Eupalopsellidae (one species), Bdellidae (one species), Cunaxidae (one species), Eupodidae (one species), Tydeidae (3 species) and Tarsonemidae (one species). Suborder: Astigmata was represented by two families: Acaridae (two species) and Glycyphagidae (one species). Suborder Cryptostigmata was represented by two families: Oppiidae (one species) and Oribatulidae (one species). On the other hand, order: Acariformes include one suborder: Mesostigmata, which represented by three families: Phytoseiidae (4 species), Ologamasidae (one species) and Ameroseiidae (one species).
https://eajbsa.journals.ekb.eg/article_17734_9dfe9b2452015d7571843205417c2fbd.pdf
Mites’ fauna – survey – taxonomy- fruit trees – Ismailia
Egypt
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
83
88
10.21608/eajbsa.2018.17735
17735
Original Article
Population Fluctuations of The Phytophagous Mite, Oligonychus mangiferus and its Predator on Mango Trees in Ismailia Governorate, Egypt
Neivin Hussian
1
Hamdy El-Sharabasy
2
Ahmed Aboughalia
ahaboghalia@yahoo.com
3
Maha Soliman
maha_soliman@hotmail.com
4
Plant protection Research Institute, Agricultural Research Center, Dokki, Egypt
Plant protection Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
Zoology Department, Faculty of Science, Suez Canal University, Egypt
Zoology Department, Faculty of Science, Suez Canal University, Egypt
Seasonal abundance of the phytophagous mite Oligonychus mangiferus and its predator Amblyseius swirskiiassociated with mango orchard trees was investigated at the experimental farm of the agriculture faculty of the Suez Canal University, Ismailia, Egypt. This study was carried out during two successive years from October 2014 to September 2016. Results showed that the temperature and moisture R.H.% were significantly affected the mean abundanceof the phytophagous mite O. mangiferus and its predator A. swirskiiassociated with mango orchard trees. The highest mean abundance of O. mangiferus was recorded in the summer season (2.96 ± 0.6 indv./leaf) and (1.33 ± 0.05 indv./leaf) for A. swirskii, respectively in the first year (from October 2014 to September 2015). On the other hand, the lowest mean abundance was recorded in the autumn (0.6 ± 0.11) for O. mangiferus, (0.23 ± 0.09 indv./leaf) for A. swirskii. The mean abundance of O. mangiferuspopulations increased to (3.43 ± 0.7 indv./leaf) in the second year of study in contrast to the populations of its predator. The results stated that the temperature had a significant effect on the population abundance of the phytophagous mite, O. mangiferus and its predator while the relative humidity had a negative effect on both populations.
https://eajbsa.journals.ekb.eg/article_17735_7675135a418197167556249c2c01a7ce.pdf
Mites
Oligonychus mangiferus
Amblyseius swirskii
Mango
Temperature
relative humidity
Abundance
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
89
97
10.21608/eajbsa.2018.17736
17736
Original Article
Study the Effect of Treated Schefflera Plants by Triacontanol Hormone (TRIA) on The Infestation by Macrosiphum euphorbia Under Glasshouses Conditions
Emam S.
1
Gamila Heikal
2
El-Roby S.
3
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Experiments were carried out to study the effect of treated schefflera plants Schefflera arboricola fam: Araliaceae by triacontanol hormone (TRIA) in different concentrations on the infestation by Macrosiphum euphorbia under glasshouses conditions.
Experiments were done in two locations International Garden, Cairo Governorate and El-Orman Garden, Giza Governorate throughout 2017 season under glasshouses conditions. Three treatments (concentrations) of triacontanol hormone were done in both two locations, small concentration (35ppm), medium concentration (50ppm) and high concentration (65ppm), beside fourth treatment which did not treat with any hormone (control). The infestation by Macrosiphum euphorbia in both two locations was the same trend. But Schefflera plants which treated with small and medium concentration of (TRIA) (35ppm, 50ppm) respectively were lower infestation by Macrosiphum euphorbia comparing to control. On the other hand Schefflera plants which treated with high concentration of (TRIA) (65ppm) were higher infestation by the same insect comparing to control. Also, experiments were done to study the effect of different concentrations of (TRIA) on the morphological and physiological adjectivesoftreated Schefflera plants. Small and medium concentrations of (TRIA) improved morphological and physiological adjectives of treated Schefflera plants. On the other hand, the high concentration of (TRIA) jaeal morphological and physiological adjectives of Schefflera plants worse than control.
https://eajbsa.journals.ekb.eg/article_17736_75fb6f3cc989c2474e99fbb3771f6140.pdf
Schefflera plants. triacontanol hormone
Macrosiphum euphorbia
glasshouses conditions
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2018-08-01
11
4
99
108
10.21608/eajbsa.2018.17737
17737
Original Article
Age Distribution Patterns of Mite ,Some Predator and Piercing Sucking Insects Inhabiting Faba Bean as A Method for Prediction of Reproductive Capabilities and Their Relationships to Phenols Leaf Content
Malakah Elsadany
malkahelsadany@gmail.com
1
Mohamed Abd El-salam
2
Acarology of Cotton and Crops Department, Plant Protection Research Institute, Sakha Agricultural Research Station, Kafr El-Sheikh, Agricultural Research Center, Giza, Egypt.
Cotton Preservation Department , Cotton Research Institute, Agricultural Research Center, Giza, Egypt.
Faba bean (Vicia faba L.) is the main source of plant proteinsin Egypt. Thiscrop is attacked by numerous of pests as mites , Aphids ,white fly and leaf hoppers. Unfortunately these pests contribute to transmit viral diseases . The experiment was carried out Sakha Agricultural Research Station , Kafr El-Sheikh Governorate and conducted in 2013/2014 season to determine theage distribution patterns of pests and predators as a method for predicting the reproductive capabilities and probability of the continuation of arthropods species in the future .In addition tothe relationships between phenols leaf contentand occurrence of arthropods was studied. Populations of sucking pests and their natural enemies on leaflets of different cultivars of faba bean; Giza 716, Sakha 3 and Giza 40 were recorded.
Theage distribution patterns of the above mentioned arthropods were represented by three patterns are expanding population, decline population and stationary population. Decline age distribution was found for phytophagous mite; Tetranychus urticae Koch on Giza 716 cultivar which means that the mite population is decreasing . The population of age distribution of Aphis Giza 716 spp. stages appeared as expanding population on the three faba bean cultivars. The population of age distribution of Empoasca sp. was expanding on Giza 716 and Giza 40 cultivars of faba bean ,but it declining on Sakha 3 cultivar. However the three age patterns were found for Chrysoperla carnea Steph., expanding for Aphidoletes aphidimyza (Rond.). Data revealed that the correlation between total phenols in faba bean leaflets were significantly positive only in Sakha 3 cultivar .
https://eajbsa.journals.ekb.eg/article_17737_872c7e1636e72e0e081620b8f0bdac03.pdf
Age distribution
Mites
sucking insects
predator insects
crops
relationship
Total phenols