eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
1
8
10.21608/eajbsa.2019.27734
27734
Original Article
Fungal Infection Causes Serious Effects on Cuticle and Midgut of the Greater Wax Moth, Galleria mellonella
Sanaa .Ibrahim
sanareda@yahoo.com
1
Taha A.
mostafataha1@yahoo.com
2
Hend Salem
hendhelmy857@yahoo.com
3
Department of Crop Pests, Plant Protection Research Institute, A.R.C.,7 Nadi El-Seid Street, Dokki, Giza, Egypt
Zoology Department, Entomology branch, Faculty of Science, AL-Azhar University, Girls Branch, Nasr City, Egypt
Zoology Department, Entomology branch, Faculty of Science, AL-Azhar University, Girls Branch, Nasr City, Egypt
The pathogenicity efficiency among two of the entomopathogenic fungi (EPF), Beauveria bassiana and Metarhizium anisopliae against the last larval instar of the greater wax moth (GWM), Galleria mellonella L., under laboratory conditions was compared. The histopathological changes in cuticle and midgut at 48hrs post fungal infection was investigated. The results revealed that, the concentration–mortality relationship showed that larval mortality increase in a linear relationship with conidia concentration and B. bassiana causing higher mortality percentage with LC50 3.1x102conidia/ml while LC50 of M. anisopliae reached 4.6x103conidia/ml. The histological analysis of B. bassiana infection at 48 hrs. post-infection showed extensive histopathological changes in cuticle and midgut that induced nodule formation beneath the cuticle layer and destroying midgut epithelium by separation of columnar layer towards gut lumen with increasing the number of regenerative cells beneath it.
https://eajbsa.journals.ekb.eg/article_27734_fb4dc10b9bc8d6c4e3a15914527368dc.pdf
Entomopathogenic fungi
Beaveria bassiana
Metarhizium anisopliae
Galleria mellonella
mortality
histopathology
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
9
18
10.21608/eajbsa.2019.28789
28789
Original Article
The Biological study of the Red Palm Weevil, Rhynchophorus ferrugineus through all a year seasons
Mohammed Aly
1
Khaled Osman
2
Mahmoud Soliman
3
Evon Hanna
yvonhanna@yahoo.com
4
Department of Zoology, Faculty of Science, South Valley University
Department of Zoology, Faculty of Science, South Valley University
Department of plant protection, Faculty of Agriculture, Bany Sweaf University
Department of Zoology, Faculty of Science, South Valley University
The most dangerous insect to threaten palm trees is the red palm weevil, Rhynchophorus ferrugineus (Curculiondiae: Coleoptera). This study was aimed to, study the Biology of the red palm weevil on the food circles (date palm, sugar cane, cucumber, banana peel and watermelon peel) under room temperature through one year. The results showed that spring was the most active season for the red palm weevil and decreases during winter. The previous plants showed their effectiveness in the breeding of the red palm weevil and the watermelon peel was nearly as close to the results of date palms (the main host of the red palm weevil), while the lowest on cucumber. The number of eggs on date palms during spring and winter were (392 and 103 eggs/female), respectively. The number of eggs on watermelon peel during spring and winter were (315 and 90 eggs/female), respectively. The number of eggs on cucumber during spring and winter were (151 and 63 eggs/female), respectively. The number of larval instars on date palm during spring and winter were (12 and 8 instars/larva), respectively. The number of larval instars on watermelon peel during spring and winter were (11 and 4 instars/larva), respectively. The number of larval instars on cucumber during spring and winter were (8 and 3 instars/larva), respectively. Pre-egg laying period on date palm and watermelon peel during spring and autumn were 3 and 4 days, respectively. Pre-egg laying period on cucumber during spring and autumn were 4 and 5 days, respectively. Based on these results must protect palms during period activity of the red palm weevil. Take in consideration, watermelon peel as a type of traps to the red palm weevil in palm plantations..
https://eajbsa.journals.ekb.eg/article_28789_1df297b21d0b7121ff99a3295aa47f63.pdf
The red palm weevil
date palm
watermelon peel
Cucumber
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
19
33
10.21608/eajbsa.2019.28791
28791
Original Article
Antimicrobial and Antiviral Activity of Lucilia sericata, Chrysomya albiceps (Diptera: Calliphoridae) And Musca domestica (Diptera: Muscidae) Whole Body Extract
Moneer Amer
1
Kotb Hammad
2
Ahmed Shehata
3
Ahmed Hasballah
4
Mahmoud Zidan
ahmedzeinhom00@gmail.com
5
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
Department of Zoology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, Egypt
The present study dealt with evaluating the effect of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica as exhibited antibacterial activity against some species of bacteria. They were shown to inhibit the growth of Gram–positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, Gram–negative bacteria, Escherichia coli and Klebsiella pneumoniae and Fungi, Aspergillus fumigatus thus exhibited limited inhibitory effect towards Gram–positive bacteria Bacillus subtilis and Gram–negative Pseudomonas aeruginosa and Fungi Syncephalastrum racemosum and Candida albicans, this effect was slowed down when challenged with secretion on a solid media but no zone of complete inhibition was detected. Growth inhibiting activity was determined in liquid growth media using the Gram–positive, Gram–negative bacterial and fungal strains as indicator organisms. The antiviral activity of whole body extracted by ethyle acetate of L. sericata, C. albiceps and M. domestica nES were tested against Vero cell line was developed for isolation and propagation of many enteroviruses and hepatitis A and average values were considered.
https://eajbsa.journals.ekb.eg/article_28791_a45bcc4e26b6cd0fe3683a126fd44584.pdf
antimicrobial activity
Antifungal activity and Antiviral activity
whole body extracts
Lucilia sericata
Chrysomya albiceps
Musca domestica
cytotoxic
VERO cell line
MTT
hepatitis A virus
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
35
43
10.21608/eajbsa.2019.29129
29129
Original Article
Feeding of the Red Palm Weevil, Rynchophorus ferrugineus Olivier (Coleoptera: Curculionidae) Larvae on Natural and Artificial Diets in Relation to Biology and Chemical Contents.
Naglaa Abdel-Hameid
naglaafkry@yahoo.com
1
Plant Protection Dept., Faculty of Agriculture, Benha Univ., Egypt
Life parameters including pre-oviposition, oviposition and larval & pupal periods, adult male and female longevities ,and generation period were recorded for the red palm weevil, Rhynchophorus ferrugineus Olivier reared on the sugarcane stem pieces (Diet,1) and other 3 artificial diets depending mainly on ground sugarcane ,(diet 2); ground corn , (diet 3) and ground mixture of sugarcane and corn (diet 4) . In the laboratory at 28±1°C and 75±5% R.H. , the effects of all diets on total proteins , carbohydrates and lipids were also determined in the last instar larvae .Larvae fed on diet 2 ( ground sugarcane + additives ) exhibited shortest larval duration ( 86.30 days ) , while diet 3 ( ground corn + additives) caused the longest larval period ( 128.35 days ) . Shortest and longest pupal periods resulted also from feeding on diet 2 and diet 3 ( 15.3 and 21.75 days , respectively ) . On the other hand , male and female longevities were the longest and shortest when previous larvae were fed on diet 2 and diet 3 ( 50.4 and 38.4 days for males and 48.0 and 36.8 days for females , respectively . The sex – ratio was almost 1:1 with all diets , except diet 2 which led to more females ( 1 male : 1.5 female ).Eggs of R. ferrugineus hatched after about 4 days , but this period was , significantly , shorter ( 3.35 days ) by rearing on diet 2 . Highest hatch ability percentage ( 93.61 % ) resulted from rearing on the same diet .
Chemical analyses of last larval instar content indicated that the highest total contents of proteins ( 51.87 mg / g ) , carbohydrates (105.0 mg / g ) and lipids ( 21.13 mg / g ) were obtained by rearing on diet2, diet 1 and the control ( natural feeding on palm ), respectively
https://eajbsa.journals.ekb.eg/article_29129_19ab0048544a0708dd6adf340dbb22d2.pdf
Red palm weevil
Artificial diets
biology
chemical contents
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
45
57
10.21608/eajbsa.2019.29178
29178
Original Article
Comparative Assessment of Certain Insecticidal Compounds on the Development and Adult Performance of Red Palm Weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae).
Tanani .A.
m.tanani2018@gmail.com
1
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Madinat Nasr, Cairo, Egypt
The red palm weevil Rhynchophorus ferrugineus is a serious pest of date palm in different regions of the Middle East. The present study was carried out to evaluate comparatively the effects of pyriproxyfen (insect growth regulator), neemazal (a plant product) and spinetoram (a bacterial product) on growth, development and adult performance of this insect pest. The early 5th instar larvae were treated with five sublethal concentrations of each compound. The most important results can be summarized as follows. Both larval duration and weight were considerably reduced by Spinetoram. Pyriproxyfen significantly reduced the larval duration only at 4000 ppm (7.2±2.97, compared to 11.7±1.6 days control insects) but Neemazal could not exhibit a remarkable effect. The strongest reducing effect on larval weight was recorded for Pyriproxyfen, since the growth inhibition% was determined as 49.10, 48.58 and 38.04, at 4000, 2000 and 1000 ppm, respectively. Pupation was inhibited by all tested insecticidal compounds and the pupal duration was significantly shortened, except at the lower three concentrations of Neemazal. Some deformations had been recorded only in adults, irrespective of the tested compound. The adult longevity was significantly shortened at the higher concentrations of each compound.
https://eajbsa.journals.ekb.eg/article_29178_b61cd43de4306baea3b221aa2bde2294.pdf
Adult
Development
Growth
longevity
morphogenesis
neemazal
mortality
Pyriproxyfen
Spinetoram
pupa
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
59
83
10.21608/eajbsa.2019.29179
29179
Original Article
Disruptive Effects of Certain Chitin Synthesis Inhibitors on Some Haemogram Parameters in the Egyptian Cotton Leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae)
Basiouny A.
ahmadlotfybasiouny@yahoo.com
1
Waheeb H.
2
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt
Department of Zoology and Entomology, Faculty of Science, Al-Azhar University, Cairo, Egypt
The Egyptian cotton leafworm, Spodoptera littoralis, is a key pest attacking several economically important crops in Egypt and several parts in the world. The objective of the present study was to investigate the effects of novaluron and cyromazine, chitin synthesis inhibitors, on the larval haemogram of this pest. Five main types of the circulating hemocytes, viz., plasmatocytes (PLs), granulocytes (GRs), prohemocytes (PRs), oenocytoides (OEs), and spherulocytes (SPs) had been categorized in last instar larvae. The most important differentiating characters of each type were described. After treatment of the freshly moulted penultimate instar larvae with LC50 of novaluron or cyromazine (2.71 and 74.44 ppm, respectively), the successfully moulted last (6th) instar larvae were used to assess the haemogram responses. Novaluron remarkably induced the total hemocyte counts (THCs) at two limits of the larval instar. In contrast, cyromazine exhibited a general inhibitory effect on THC during the majority of larval instar. As a response to novaluron treatment, PRs and PLs counts had been slightly induced during the first half of instar but slightly reduced during the second half. Also, GRs population decreased but SPs population increased along most larval period. With respect to the effect of cyromazine, count of PRs and GRs had been remarkably inhibited but PLs was significantly enhanced along the larval life. The same compound exhibited diverse effects on SPs, depending on the larval age. In addition, OEs count was enhanced by both compounds during the second half of larval instar. Both compounds exhibited serious cytopathological effects on all types of hemocytes, except cyromazine against OEs.
https://eajbsa.journals.ekb.eg/article_29179_18bee2eb94350d49b225278bab49da1f.pdf
granulocyte
hemocyte
Haemolymph
histopathology
oenocytoid
Plasmatocytes
prohemocyte
spherulocytes
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
85
97
10.21608/eajbsa.2019.29777
29777
Original Article
Review of Subfamily Palparinae (Neuroptera: Myrmeleontidae) from Egypt
Hayam El Hamouly
1
Rabab Sawaby
rababsawaby@sci.asu.edu.eg
2
Gawhara Abu El-Hassan
gawhara_magdy@hotmail.com
3
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
Entomology Department, Faculty of Science, Ain Shams University, Abbasia, Cairo Egypt
Department of Entomology, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
The Egyptian fauna of subfamily Palparinae is revised. Four genera and nine species are recognized. A key to genera and species with illustrations is provided. Diagnosis, specimens examined and distributions are given for each species.The Egyptian fauna of subfamily Palparinae is revised. Four genera and nine species are recognized. A key to genera and species with illustrations is provided. Diagnosis, specimens examined and distributions are given for each species.
https://eajbsa.journals.ekb.eg/article_29777_52b8ba87d6cc52624dbf767ac5fde48d.pdf
Antlions
Diagnosis
Palpares
Parapalpares
Egypt
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
99
108
10.21608/eajbsa.2019.30407
30407
Original Article
Impact of Some Weather Factors on the Population Density of Phenacoccus solenopsis Tinsley and its Natural Enemies
Hassan Nabil
1
M. Hegab
hegabmohamad@gmail.com
2
Plant Protection Research Institute, ARC, Dokki, Giza, Egypt
Plant Protection Dept., Faculty of Agric., Zagazig Univ., Zagazig, Egypt
The aimed of this investigation was to estimate the seasonal abundance and activity periods of the cotton mealybug, Phenacoccus solenopsis Tinsley and its associated parasitoid on okra plants. The present experiment was conducted at Hihhya distract, Sharkia Governorate during two consecutive seasons 2017 and 2018. Plant samples were taken weekly to calculate the population density of different stages of tested pest and its parasitoid, the effect of some weather factors, the number of generations and the best leaf surface. The present work was found a reverse relation between the populations of the cotton mealybug (CMB) and its associated parasitoids. The population dynamic of CMB was high while the population of its parasitoids was low in the first season. On the other hand, the opposite results were obtained during the second season. The P. solenopsis had two to three generations during the first and second seasons, respectively. There was significantly positive correlation between maximum temperature and the population females of this insect whereas, a significant negative correlation was found with mean of relative humidity during the period of this investigation.The results of this study may help the researchers for designing a comprehensive pest management program and prediction models for the cotton mealybug to restrict their further spread.
https://eajbsa.journals.ekb.eg/article_30407_224c1f57654fc8218372d50991362dd4.pdf
Cotton mealybug
seasonal abundance
Parasitoids
okra plants
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
109
126
10.21608/eajbsa.2019.30680
30680
Original Article
Taxonomical Revision on Certain Species of Subfamily Coccidulinae (Coleoptera: Coccinellidae) in Egypt
Mohamed Abied
1
Abdel-Hakam El-Saeady
2
Sherif Hafez
3
Mohammad Bedewy
bedewy80@gmail.com
4
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
This is the first paper of series reviewing the Egyptian Coccidulinae. Three genera Cryptolaemus Mulsant, Rhyzobius Stephens and Tetrabrachys Kapur belonging to two tribes Coccidulini and Tetrabrachini are revised depending on materials collected during study years, and specimens preserved in four insect collections; Collection of Alfieri, Department of Plant Protection, Faculty of Agriculture, Al-Azhar University (ALFC); Collection of Ain Shams University, Faculty of Science, Department of Entomology (ASUC); Collection of Cairo University, Faculty of Science, Department of Entomology (CUC) and Collection of the Plant Protection Research Institute, Ministry of Agriculture (MAC). Genus Cryptolaemus transferred from Scymninae to Coccidulinae, and the species Tetrabrachys cribratellus is proposed as a valid species. Keys are provided to separate the tribes, genera and species. Description is based on adults, and detailed information about distribution and some ecological notes are mentioned.
https://eajbsa.journals.ekb.eg/article_30680_12267ca329db8053960a4ec7c23f99ab.pdf
Coccinellidae
Coccidulinae
Palaearctic
Survey and Taxonomy
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
127
138
10.21608/eajbsa.2019.31524
31524
Original Article
Effect of Two Food Types on the Biological Aspects of the Predatory Mite, Coleoscirus horidula (Tseng) (Acari: Prostigmata: Cunaxidae) at Various Constant Temperatures
Khalil M.
1
Abo-Zaed E.
2
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
The cunaxid predacious mite, Coleoscirus horidula (Tseng) was extracted from leaf litter under cotton field plants in Qalyubia Governorate associated with spider mites, nematodes, scale insects as well as other small arthropods. The biological aspects of the predatory mite, C. horidula was determined on two preys, the free living nematode Rhabditis scanica Allegen and the acarid mite Tyrophagus putrescentiae (Schrank) at four different temperature degrees (20, 25, 30, 35°C) and 65-70 ± 5% RH. The predator passed through three nymphal stages, also the predator developed faster when reared at 35°C than 20°C. Obtained data showed that the type of prey and temperature affected significantly on biology of male and female of C. horidula. As it prolonged C. horidula life cycle, generation, longevity and life span when fed on free living nematodes R. scanica at 20ºC, while it was significantly decreased when fed on motile stages of acarid mite T. putrescentiae at 35ºC, as the periods decreased. Fecundity was significantly higher when the female fed on free-living nematodes recording 98.83 eggs/ female at 25°C and decreased to record the lowest number of deposited eggs at 35°C as45.67 eggs/ female on the same prey.
https://eajbsa.journals.ekb.eg/article_31524_ace6243b9603a6ee9c33087608e0de9e.pdf
predacious mite
Cunaxidae
free nematodes
biology
Acaridae
eng
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences. A, Entomology
1687-8809
2090-0813
2019-03-01
12
2
139
149
10.21608/eajbsa.2019.43053
43053
Original Article
Review of Subfamily Brachycerinae (Coleoptera: Brachyceridae) in Egypt
Neven Elmetwaly
1
Fatma Hamed
fatmazakarya111@gmail.com
2
Plant Protection Reasearch institute, Agriculture Research Center - El-Dokki-Giza
Zoology Department, Faculty of Science (Girls), Al-Azhar University
The gathered information indicated that subfamily Brachycerinae is represented in Egypt by a single genus, Brachycerus that contains 8 species. Their diagnostic characters were introduced in this work according to previous investigators. This study was planned to identify and to determine the recent taxonomic status of Brachycerides species for the first time in Egypt. An illustrated key was given for all Brachycerides species.
https://eajbsa.journals.ekb.eg/article_43053_5490094b773581fd2e41efaa731a9767.pdf
Taxonomic key
Brachyceridae
Brachycerinae
Brachycerus
Egypt