Development Rate and Ultrastructure Changes of Puparia of Megaselia scalaris (Loew) (Diptera: Phoridae) Induced by Azadirachtin.

Author

Biological and Geological Sciences Dept., Fac. of Education, Ain Shams Univ., Egypt.

Abstract

The effect of azadirachtin on the development and mortality of the scuttle fly, Megaselia scalaris (Loew) (Diptera: Phoridae) was investigated in the laboratory by using neem extract, Achook®, containing 0.15% azadirachtin. Different biological changes were recorded after treatment 1st larval instar with different concentrations of azadirachtin as larval mortality, pupation rate and adult emergence of. Azadirachtin-LC50 against the 1st instar larvae of M. scalaris was 13.79 mg/L. Exposing larvae to LC50 of azadirachtin caused many changes in the puparia such as having folded cuticle and swelling of the integument compared to control. Distortion of the anterior spiracle and papillae found on respiratory horns were also observed. The present study suggests that azadirachtin hampers the development rate of M. scalaris by reducing the insect population and causing several scanning electron microscopic changes in the puparia of the insect.

Keywords