@article { author = {Abd El-Aziz, Mona and El-Sayed, Yasser}, title = {Toxicity and biochemical efficacy of six essential oils against Tribolium confusum (du val) (Coleoptera: Tenebrionidae)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {1-11}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15424}, abstract = {          Six essential oils extracted from  garlic  (Allium sativum), mint (Mentha piperitaa), basil (Ocimum basilicum), thyme  (Thymus vulgaris ) , sesame (Sesamum Indicume) and chamomile (Chamaemelum nobile) were tested for both  repellency and toxicity  against the  confused  flour  beetle,  Tribolium confusum adults and  last larval instar. Two kinds of repellency bioassays were performed by using filter paper tests and treated kernels.  The toxicological bioassay was performed by topical application. The results showed that the tested oils were highly repellent to the adult insect compared to the larvae. By contrast, the toxicological bioassay showed that larvae were more susceptible to tested essential oils than the adults.  Effects of essential oils on five detoxification enzymes were also investigated. A reduction in total activities of the major detoxification enzymes in treated larvae was also observed.}, keywords = {Essential oils,Tribolium confusum,repellency,detoxification enzymes}, url = {https://eajbsa.journals.ekb.eg/article_15424.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15424_d42a8ae964ff035d1af5e516619a5dd2.pdf} } @article { author = {El-Kholy, Magdy and El-Saiedy, El Sayed}, title = {Biological control of Thrips tabaci (lind.) and Aphis gossypii (Glover) using different predatory Phytoseiid mites and the biocide vertimec on Eggplant at Behaira Governorate.}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {13-22}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15425}, abstract = {Three predatory mite species, Phytoseiulus persimilis (Athias-Henriot), Neoseiulus cucumeris (Oudemans) and Neoseiulus californicus (McGregor) and biocide certimec were evaluated as potential biological control agents for Thrips tabaci (lind.) and Aphis gossypii (Glover) on two eggplant cultivars in open field at Behaira Governorate. Field releases of different numbers of mites per plant during 2008 season showed that Neoseiulus cucumeris reduced the number of thrips and aphid in two cultivares of eggplant. We found that earlier releases (averages from 20-25 individuals / leaf) resulted in reducing thrips and aphids numbers and lowering their damage. We conclude that inoculative release of Neoseiulus cucumeris one of a potentially useful thrips and aphids management strategy, but needs improvements in release timing and strategy will be required to provide commercially acceptable control.}, keywords = {Phytoseiulus persimilis,Neoseiulus colifornicus,Neoseiulus cucumeris,Thrips tabaci,Aphis gossypii,Biocide,Eggplant}, url = {https://eajbsa.journals.ekb.eg/article_15425.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15425_c077aba9d750034873f95c63380858a9.pdf} } @article { author = {Ayaad, Tahany and Shaker, Ghada and Almuhnaa, Amal}, title = {Isolation of antibacterial peptides from Saudi Arabian honeybees and investigating the antimicrobial properties of natural honey samples}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {23-34}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15426}, abstract = {As part of the ongoing search for novel antimicrobial agents and their use in singular or combined drug therapy, a polypeptide fractions of Mr about 14.500 and 15.00 KDa were isolated from the lymph fluid of two groups of intact and immunized Saudi Arabian honeybees (Apis mellifera) obtained from Al-Qasim and Hail locations around Riyadh during the honey season 2008 following experimental infection with 1.1 x l06 viable Escherichia coli cells(ATCC 25922).  The polypeptide was purified to homogeneity by Reversed Phase High Performance Liquid Chromatography. Antibacterial activity of the isolated polypeptide was evaluated in vitro by an agar well diffusion method for E.  coli strain (ATCC 25922) and Klebsiella pneumoniae strain (ATCC 11678) the major Gram negative pathogens causing urinary tract infections, and Staphylococcus aureus (ATCC 6538) as Gram positive bacteria. A total of ten honey samples collected from different floral areas around Riyadh were also investigated for their antimicrobial activity against one yeast, Candida albicans (ATCC 10231) and four standards bacteriastrains, E. coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), S. aureus (ATCC 6538),  Bacillus subtilis (ATCC 6633) using standard antimicrobial assays.  The isolated polypeptide and the different honey samples revealed comparable marked variations in antimicrobial activities and their sensitivity might be depending on their floral source.}, keywords = {Antibacterial peptides,Saudi Arabian honeybees}, url = {https://eajbsa.journals.ekb.eg/article_15426.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15426_d04eabb543c8e5a5092ebd021e5b5bfb.pdf} } @article { author = {Abdullah, Mohammed}, title = {Identification of the biological active compounds of two natural extracts for the control of the red palm weevil, Rhynchophorus ferrugineus (Oliver) (Coleoptera - curculionidae)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {35-44}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15427}, abstract = {Two natural insecticides, rotenone and limonine caused antifeedant and growth inhibition of the red palm weevil Rhynchophorus ferrugineus larvae and adults. Rotenone was extracted from the root of Lanchocarpus sp. or Derris sp. and limonene extracted from citrus fruit oil. The concentrations of 1.5, 3, 6, 9, 12 and 15% of both insecticides were used for treatment of the sugar cane pieces. Adult stage was more tolerant than the larvae. Also, the rotenone insecticide was more effective than limonene insecticide. Moreover increasing of rotenone and limonene concentrations were reduced the food consumption and increased the larval and adult mortality. The chemical analysis was suggested the structure of rotenone is flavonoid compound and its chemical formula is C23H22O6. While the chemical nature of limonene is alkaloid with the chemical formula of C10H13O4.}, keywords = {The red palm weevil,natural insecticides,rotenone and limonene}, url = {https://eajbsa.journals.ekb.eg/article_15427.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15427_c993e56279d6d44275b6e83d51460331.pdf} } @article { author = {Abdullah, Mohammed}, title = {Toxicological and histopathological studies of Boxus chinensis oil and precocene II on larvae of the red palm weevil Rynchophorus ferrugineus (Oliver) (Coleoptera : Curculionidae )}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {45-54}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15428}, abstract = {Twonatural biopesticides, (Boxus chinensis oil and precoceneII) were testedagainst 10 days-old larvae of the red palm weevil, Rhynchophorus ferrugineus.The two biopesticidse had toxicological and pathological effects on Rh.ferrugineus larvae. The toxicity effect of B. chinensis was morethan precoceneII against Rh. ferrugineus.  In order of toxicity the LC50 of B.chinensis was 0.68% while the LC50 of precoceneII was 1.36%. Onthe other hand the mid-gut tissues of the 10days old larvae were exhibiteddifferent histopathological lesions due to effect of B. chinensis. The alterations included analyze and destroyed of epithelial cells, vaculation of cytoplasm and dissolve of nuclei of the epithelial cells.}, keywords = {Boxus chinensis oil,precoceneII,histopathology,biopesticide effect,Rhynchophorus ferrugineus}, url = {https://eajbsa.journals.ekb.eg/article_15428.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15428_50a91da194daa60957a3cca5c137aafb.pdf} } @article { author = {Ayaad, Tahany}, title = {Isolation of midgut agglutinin of Culex quinquefasciatus ( Diptera , Culicidae)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {55-68}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15429}, abstract = {A Lectin that agglutinates Eschrechia coli (ATCC 25922) , Staphylococcus aureus (ATCC 6538) live bacteria and various  mammalian red blood  cells (RBCs)  was identified in Culexquinquefasciatus midgut extract ( Cqlec) by using human (three groups: A, B,  and O, RH+)mouse, rat, guinea-pig, rabbit  and goat erythrocytes. With the use of (NH4)2 SO4 fractionation, anion - exchange and GluNA -CBr-Sepharose 6B affinity chromatography, C.quinquefasciatus mid gut lectin has been purified to homogeneity. IEF and reducing SDS/PAGE revealed that the isolated mid gut lectin have isoelectric point (PI) of 6.30, and a subunit approximate molecular weight of 34.5 KDa .The highest agglutination activity of crude and isolated Cqlec   were detected against both Eschrechia coli cells and rabbit RBCs. Significant differences in hemagglutinin titers and carbohydrate inhibition were detected between sugar fed and blood fed  adult female mosquitoes.Overall agglutinin levels were increased following a blood meal feeding  and E. coli induction using a hypodermic needle. This study presents the first report on the occurrence of heterogeneous anti rabbit RBC agglutinins in the midgut extracts of C.quinquefasciatus from Al kharj area in Saudi Arabia. The HA of lectins are Ca2+independent, heat-resistant, and are strongly inhibited by D(+)-mannose and D(+) glucose  followed by N-acetyl-D-glucosamine. Raffinose and N-acetyl-D-manosamine were found to be moderate inhibitors. Non of the lectins were inhibited by galactose , lactose , trehalose or fetuin (1%) but the glycosubstances mucin and laminarin showed strong inhibitition using low concentrations . 2D-NMR spectroscopy revealed a component of the corresponding residue in structure having group regions of  resolution spectrum of  Man9- GlcNAc2Asn.}, keywords = {Lectin,hemagglutination,Midgut,Culex quinquefasciatus,Mosquitoes,Culicidae,NMR}, url = {https://eajbsa.journals.ekb.eg/article_15429.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15429_3e8c8f0dcae7aa0cc31c500011dcf2bf.pdf} } @article { author = {Bakr, Reda and Badawy, Rawda and Mousa, Saber and Hamooda, Laila and Atteia, Sahar}, title = {Ecological and taxonomic studies on the scale insects that infest mango trees at Qaliobiya governorate}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {69-89}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15430}, abstract = {This study aimed to determine the recent status of the scale insects that infest the mango trees at Qaliobiya governorate during visiting four mango orchards (El-Khanka, Shebeen El-Qanater, Benha &El-Qanater El-Khayria, through twice visits monthly to each district for two successive years. This study was carried out to present an identification guide with key, diagnosis, important synonymes, figures & illustrations to   the fourteen collected mango scale pests which included in the four families, Coccidae, Pseudococcidae, Margarodidae and Diaspididae; in additions to give an attention to the most serious species, with notes on the agricultural precautions which could decrease the infestation. Mango leaf morphology and anatomy is used to explain the reasons for the mango variety recommendation.}, keywords = {Ecology,Taxonomy,scale insects,mango trees}, url = {https://eajbsa.journals.ekb.eg/article_15430.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15430_12f8d1af101b04ec44fc75b7214f4074.pdf} } @article { author = {Mohammad, Salwa and Gad Alla, Sohair and El-Hamouly, Hayam and El-Den Nasser, Mohammad}, title = {Cuticulear hydrocarbons profiles of seven common Egyptian mantis}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {91-94}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15431}, abstract = {This is the first chemical taxonomic study of cuticular hydrocarbons of seven common mantis species in Egypt. This study is shedding the light on the role of this chemicals in phylogeny and taxonomy of this charismatic group of Insects.}, keywords = {cuticuler hydrocarbons,cuticuler extractions,Empusidae,Eremiaphilidae,Mantidae,Tarachodidae,Phylogeny}, url = {https://eajbsa.journals.ekb.eg/article_15431.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15431_46d8b7ee1642c356d457cd66a3709a28.pdf} } @article { author = {Mohammad, Salwa and Fadl, Hassan and Gadalla, Sohair and Badrawy, Haitham}, title = {A revision of the genus Nemotelus Geoffroy (Diptera: Stratiomyidae) from Egypt, with one new species and three new records}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {95-110}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15432}, abstract = {The Egyptian fauna of the genus Nemotelus Geoffroy was revised.  We recognize 9 species (compared to 5 species recognized about 40 years ago). As a result of this revision N. matrouhensis sp. nov.is described as new. N. anchora Loew, 1846; N. candidus Becker, 1906 and N. oasis Becker, 1906 are newly recorded from the Egyptian fauna. The female of N. albifacies Becker, 1902 is described for the first time and both sexes of N. niloticus Olivier, 1811 are redescribed and illustrated for the first time.             N. punctiventris Becker, 1902 is removed from synonymy with N. brachystomus Loew, 1846 and N. albifacies Becker, 1902 and N. oasis Becker, 1906 are removed from synonymy with N. niloticus Olivier, 1811. Also, N. theodori Lindner, 1974 is newly synonymized with N. oasis Becker and N. brachystomus aegyptiacus Lindner, 1925 is newly synonymized with N. punctiventris Becker.             With the addition of new characters such as the head index, shape of antennae and abdominal pattern, the females of N. candidus Becker, 1906 and N. marinus Becker, 1902 are diagnosed and illustrated for the first time. In addition, illustrations, keys, specimens examined, geographical and local distribution are given for each species.}, keywords = {Taxonomic revision,new species,New records,Nemotelus,Egypt}, url = {https://eajbsa.journals.ekb.eg/article_15432.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15432_e5af20fe8fda392c642cd3fbdd06dae3.pdf} } @article { author = {Moustafa, Sameh}, title = {Response of the Mediterranean fruit fly, Ceratitis capitata (Wied.) and peach fruit fly, Bactrocera zonata (Saund.) to some food attractants}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {111-118}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15433}, abstract = {The mean captured adults of the Mediterranean fruit fly (MFF), Ceratitis capitata (Wiedemann) per trap per day (CTD) in the traps containing Glan, Pro-lure 2%, Agrisense, Bioprox, Pro-lure 5%, Amadene, Buminal, Norlan and Agrinal were 11.04, 10.55, 10.22, 7.62, 6.56, 3.98, 3.16, 2.98 and 1.89, respectively. While, the effectiveness of the tested food attractants against the peach fruit fly (PFF),  Bactrocera zonata (Saunders) adults comes in descending order as follows: Gla > Pro-lure 5 > Pro-lure 2% > Bioprox > Agrisense > Agrinal > Buminal > Norlan and Amadene; however, the CTDs of these preparations were 0.60, 0.60, 0.51, 0.49, 0.42, 0.22, 0.15, 0.13 and 0.13, respectively. The present results showed that, adding the pesticide, malathion to the food attractant preparations was obviously reduced the attractiveness of the lures to both MFF and PFF adults. All of the tested preparations were attracted MFF and PFF females with a significantly high numbers in comparison to males. Regression analysis illustrated that the tested food attractants exhibited high stability by the time passed, where the passed time had not any significant effect on the potentiality of the tested preparations.}, keywords = {Mediterranean Fruit Fly,Ceratitis capitata,Bactrocera zonata,food attractants}, url = {https://eajbsa.journals.ekb.eg/article_15433.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15433_258a42e333ac134160c38f6d28bdb020.pdf} } @article { author = {Mohamed, Gamal El-Dein and Ibrahim, Sanaa and Moharum, Fatma}, title = {Effect of Saccharicoccus sacchari (Cockerell) infestation levels on sugarcane physical and chemical properties}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {119-123}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15434}, abstract = {The present investigation was carried out in Sohage   Governorate, Egypt on sugarcane infested with Saccharicoccus sacchari (Cockerell) (Hemiptera: Pseudococcidae) during growing year 2007/2008. Obtained results indicated that only sever infestation significantly affected sugarcane physical and chemical properties. There were reduction of stalk's weight (5.03, 34.33%), stalk's height (2.16, 6.93%), stalk internodes' number per stalk (8.8, 29.07) and juice weight (2.26, 31.62%) in low and heavy Infestation, respectively compared with infestation free. Internodes height increased by 8.59 and 35.45% due to low and sever levels of infestation, respectively. There were losses of brix (4.95, 13.47%), sucrose (6.29, 27.87%), purity (1.41, 16.87%), pol (5.29, 32.6%) and sugar recovery (5.29, 37.08%) and increase of reducing sugar (3.53, 7.68%) and fiber (1.28, 3.85%) due low and heavy levels of infestation, respectively.}, keywords = {Saccharicoccus sacchari,level of infestation,physical and chemical properties of sugarcane}, url = {https://eajbsa.journals.ekb.eg/article_15434.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15434_0f98aa533cd5a348ca6b5f47be004735.pdf} } @article { author = {Ebrahim, Ayman}, title = {Taxonomic position of Order Diptera in Egypt}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {125-131}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15435}, abstract = {According to the changes occurred in the classification of the categories of the order Diptera due to the recent studies which based on modern taxonomy methods, using molecular characters, phylogeny and cladistic analysis, the present work presenting the taxonomic position and higher classification of order Diptera in Egypt based on the most recent investigation made with modern methods, together with morphological characters, to update and correct the Egyptian list of the order Diptera.  }, keywords = {Diptera,classification,Diopsidae,Egypt}, url = {https://eajbsa.journals.ekb.eg/article_15435.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15435_ee0c6b868f5476981b0f498c75dd702d.pdf} } @article { author = {Ebrahim, Ayman}, title = {Described a new recorded family Diopsidae of (Order Diptera) with its Species Diopsis apicalis in Egypt}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {135-145}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15436}, abstract = {During rearranging and trying to identify the unidentified specimens of the order Diptera in the main reference insect collection of the Plant Protection Research Institute, 25 unidentified dipterous specimens that were collected from Armant (Assiut, Egypt) attracted the attention with its eyes that far projected from the head. These specimens were identified to the family rank (Diopsidae) by using the key. The representative specimens of this family were identified by Prof. Dr. Hans Feijen to the species ( Diopsis apicalis ).             The present study includes Description and taxonomic characters of the family and its species with illustrated species}, keywords = {Diptera,Diopsidae,Diopsis,Diopsis apicalis Distribution,Egypt}, url = {https://eajbsa.journals.ekb.eg/article_15436.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15436_de76c00a888745f76f2e84a621ab5251.pdf} } @article { author = {S., Ghoneim and . A., Tanani and L., Basiouny}, title = {Influenced survival and development of the desert locust Schistocerca gregaria (acrididae) by the wild plant Fagonia bruguieri (zygophyllaceae).}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {147-164}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15437}, abstract = {Three extracts were prepared from the wild plant Fagonia bruguieri: methanolic extract, petroleum ether extract and n-butanolic extract. These extracts were assessed against the penultimate and last instar nymphs of Schistocerca gregaria.  After treatment of the penultimate instar nymphs, a dose-dependent trend of mortality could be observed for the methanolic extract. To some extent, a lesser toxic action was exerted on the nymphs by petroleum ether extract or n-butanolic extract. After treatment of the lastinstar nymphs, an ascending mortality % was estimated as the concentration level of methanolic extract was increased. After treatment of the penultimateinstar nymphs, the growthof the same treated nymphs was affected to some extent by the methanolic extract, irrespective of the concentration level. The remarkably influenced nymphal growth was detected only at the highest concentration level of petroleum ether extract and the higher two concentration levels of n-butanolic extract. Also, the profoundly extended developmental duration was caused by petroleum ether extract at the higher three concentration levels.  After treatment of the last instar nymphs, all extracts exhibited inhibitory effects on the growth because the nymphs were prohibited to gain somatic weights as their control congeners obtained. A fastening action of methanolic extract on the developmental rate along shortened developmental duration especially at the highest concentration level. In contrast, a retarding action on such rate of the developing nymphs was exerted by both the petroleum ether extract and n-butanolic extract. The Fagonia bruguieri extracts intervened in the metamorphosis program because some nymphal-adult intermediates were formed.}, keywords = {Schistocerca gregaria,Fagonia bruguieri,Lethality,Growth,Development,nymph,weight gain,nymphal-adult intermediates}, url = {https://eajbsa.journals.ekb.eg/article_15437.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15437_2513850649e52faa1ca7604a2284caa3.pdf} } @article { author = {Abd-Rabou, Shaaban and Ali, Nadia and El-Fatih, Monira}, title = {Life table of the hemispherical scale, Saissetia coffeae (Walker) (Hemiptera: Coccidea)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {165-170}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15438}, abstract = {The hemispherical scale, Saissetia coffeae (Walker) (Hemiptera: Coccidae) is one of the most importantpest attacking olive trees in Egypt.The aim of the present work is to study the biological studies of thehemispherical scale, S. coffeae. Mean durations of the first instar were15.5± 0 .85, 12.8 ± 0.63 and 9±0.76 days at 18, 24 and 30oC,respectively. Second instar lasted for 23.3±0.67, 20.6±0.52 and 16.9± 0.88days, respectively. While third instar durations were 22.6± 0.70, 19.8±0.63 and13.2± 0.79, respectively.  Incubationperiods were 21.4 ± 1.17, 14.4 ± 0.70 and 10.1 ± 0.88 days, respectively. Thegeneration time was 82.80±1.48, 67.6±1.26 and 49.2±2.10 days, respectively. Asa result the durations of the adult longevity were 6±0.67, 8.7±0.82 and 8.9 ±0.74 days at 18, 24 and 30oC, respectively.}, keywords = {life table,Saissetia coffeae,Hemiptera}, url = {https://eajbsa.journals.ekb.eg/article_15438.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15438_cee661d45436d1c03b7212534f970db7.pdf} } @article { author = {AREF, Noeman}, title = {Effect of Bermuda Grass Cynodon dactylon Extracts on Cotton Leaf Worm, Spodoptera littoralis Boisduval (Lepidoptera: Noctuidae)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {171-175}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15439}, abstract = {Bermuda grass, Cynodon dactylon (L.) extracts were tested on cotton leaf worm, Spodoptera littorals (Boisd.). Five solvents were used in preparing of these extracts, (i.e., methanol, petroleum ether, acetone, diethyl ether and distilled water). Distilled water was used as polar solvent. The results showed that extracts of non polar solvents were more effective than water. Methanol extract resulted in highest number of giant larvae. Methanol and diethyl ether extracts resulted in the highest number of deformed pupae. Acetone extract caused highest number of mortalities, followed by diethyl ether and methanol extracts. Methanol extract proved to be the most efficient on fertility of S. littorals. All extracts had a slight effect on the larval and pupal stages duration compared with control. Larval and pupal weights were affected in the same manner also. It was concluded that all extracts caused a disturbance in hormonal balance in the larvae.  }, keywords = {Spodoptera littoralis,Cynodon dactylon}, url = {https://eajbsa.journals.ekb.eg/article_15439.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15439_cd66f91379cc4cc61b8a9b4393b9337f.pdf} } @article { author = {Moustafa, Sameh and Abd El-Mageed, Ahmed and El-Metwally, Mostafa and Ghanim, Nabil}, title = {Efficacy of Spinosad, Lufenuron and Malathion against olive fruit fly, Bactrocera oleae (Gmelin) (Diptera: Tephritidae)}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {171-178}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15440}, abstract = {Spinosad, Lufenuron and Malathion were evaluated in controlling olive fruit fly, Bactrocera oleae (Gmelin) under field conditions by using partial bait spray and killing bags during fruiting seasons of 2008 and 2009. The obtained results showed that the percentages of B. oleae populations and fruit infestation were obviously low in treated plots with Lufenuron, Spinosad and Malathion, respectively in comparison with control plot which were relatively high. The mean reduction percentages in B. oleae population in treated plots with Lufenuron, Spinosad and Malathion were 86.2±8.2, 77.3±3.0 and 71.3±11.2% during 2008 season and 74.8±10.1, 71.9±9.4 and 70.2±7.2% during 2009 season, respectively. While, the mean reduction percentages in fruit infestation by B. oleae larvae in treated plots with Lufenuron, Spinosad and Malathion were 77.5±6.5 & 73.1±5.1, 76.9±5.2 & 71.5±5.1 and 70.8±6.8 & 64.9±6.2% during 2008 & 2009 seasons, respectively.}, keywords = {Bactrocera oleae,Spinosad,lufenuron,malathion,partial bait spray}, url = {https://eajbsa.journals.ekb.eg/article_15440.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15440_c88794ffdf88d5de6a2b5a26757833b9.pdf} } @article { author = {El-Gindy, Mohamed and El-Refaey, Rasha and Abou Hatab, Eftkhar}, title = {Abundance of some potato homopterous pests as affected by potassium fertilization level}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {179-185}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15441}, abstract = {Experiments were conducted during potato winter seasons of 2007/08 and 2008/09 at Dakahlia, Egypt. Four potassium fertilization levels (0, 100, 150 and 200 kg/fed) were applied to clarify their effects on the population density of Myzus percicae (Sulzer), Aphis gossypii Glover, Aphis craccivora Koch, Bemisia tabaci (Gennadius) and Empoasca sp. in potato fields. The obtained results showed that the highest population densities of these pests occurred at no potassium fertilization (control) followed by 100 kg/fed and 150 kg/fed, respectively. The lowest population densities were recorded at 200 kg/fed treatment. A. gossypii and M. persicae were the most affected pests by potassium fertilization. A. craccivora and Empoasca sp. were affected in relatively moderate way. B. tabaci was the least affected one. On the other hand, epidermal thickness of potato leaves was significantly increased by fertilization level increase.  }, keywords = {potassium fertilization,potato,Myzus percicae,Aphis gossypii,Aphis craccivora,Bemisia tabaci,Empoasca sp. abundance}, url = {https://eajbsa.journals.ekb.eg/article_15441.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15441_23f24d25beafbf324fbb021f6e025abd.pdf} } @article { author = {Galal, Fatma}, title = {Comparison of RAPD and PCR-RFLP markers for classification and taxonomic studies of insects}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {187-195}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15442}, abstract = {            Experiments were conducted to assess the efficiency of RAPD and PCR-RFLP marker systems as molecular tools for taxonomy and classification of insects. Three species of adult ants, Camponotus maculatus, Monomorium pharoensis and Cataglyphis bicolor, were used in this study. Total genomic DNA was extracted and used for PCR amplification of the ITS region. The purified PCR products were subjected to single digestion with EcoRI, MspI, TaqI and SacI enzymes and visualized on 2% agarose gel. In addition, 40 arbitrary primers were used to generate RAPD-PCR profiles. Data obtained from RFLP, RAPD and RFLP+RAPD were statistically and phylogenetically analyzed to determine the level of polymorphism in each case. Intraspecific, interspecific variation levels and phylogenetic relationships were investigated. Results were discussed, comparatively, and the final conclusion was: on the bases of ease, cost, technical labor, speed and amount of DNA needed, the RAPD marker system is preferentially used in taxonomic and classification studies. On the bases of degree of polymorphism, precision of genetic distance estimates and the statistical power of the test, no differences were observed between RAPD and PCR-RFLP tests.}, keywords = {PCR-RFLP,RFLP,RAPD-PCR,RAPD,polymerase chain reaction}, url = {https://eajbsa.journals.ekb.eg/article_15442.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15442_d8cf53d136338ff05be35705e9c2072b.pdf} } @article { author = {Hassan, Heba}, title = {Efficiency of some new insecticides on physiological, histological and molecular level of cotton leafworm.}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {197-209}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15443}, abstract = {Efficiency of indoxacarb, spinetoram and methoxyfenozideon cotton leaf worm (Spodoptera littoralis) was determined throughexposure of second and fourth instar larvae to dipped castor bean leaves. LC50estimates of second instar larvae ranged from 0.004 to 0.006 ppm of indoxacarb,0.022 to 0.033ppm of spinetoram and 0.006 to 0.011ppm of methoxyfenozide. LC50estimates of fourth instar larvae ranged from 0.36 to 0.54 ppm of indoxacarb, 1.78to 2.64ppm of spinetoram and 1.36 to 1.69 ppm of methoxyfenozide. Also, theovicidal activity of these compounds was studied. Three day old eggs are moreaffected than that of one or two days old in case of indoxacarb and spinetoramwhile the reverse was in the case of methoxyfenozide. In nutritional assays,all tested compounds reduce food intake and affected growth rate (GR) whereindoxacarb caused the highest rate of efficiency and followed bymethoxyfenozide and spinetoram  against 4thlarval instar fed for two days on castor leaves treated with LC50sof the previous coumpounds. The histological examinations of 6thlarval instar cuticle (after treatment of fourth larval instar by LC50of methoxyfenozide) showed destruction in the cuticle layers, fissures in theendocuticle and irregular distribution of the hypodermal cells. Whileindoxacarb and spinetoram treatments showed slight effect in the cuticle layersas compared to methoxyfenozide.             In addition, the deteriorativepotentialities of aldehyde oxidase and α -glycerophosphate dehydrogenase pattern of S.littoralis were screened.}, keywords = {Indoxacarb,Spinetoram,Methoxyfenozide,Spodoptera littoralis,ovicidal effect,food consumption,histopathology,cuticle,Aldehyde,oxidase,α – glycerophosphate,dehydrogenase,pattern}, url = {https://eajbsa.journals.ekb.eg/article_15443.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15443_ae7b8134e9bba6ff510c1fba25b4e335.pdf} } @article { author = {Abd El-Gawad, Hany and Abd El-Aziz, Abd El-Aziz and Sayed, Atef}, title = {Effect of releasing the parasitoid Anisopteromalus calandrae (Hymenoptera: Pteromalidae) on certain coleopteran stored products beetles in Egypt}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {211-219}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15444}, abstract = {The effect of direct release (inside bags) of faba bean seeds or wheatgrains with the release ofAnisopteromalus calandrae was studied on insects populations, percentage damage seeds and percentage weight loss of stored seeds or grains due to infestation with Callosobruchus maculates (F.), Callosobruchus chinensis (L.) on faba bean seeds, Rhyzopertha dominica (F.)and Sitophilus oryzae (L.) on wheat grains at Ismalia and Sharkia governorates locations. Results showed release of A. calandrae reduced the monthly rate of increase of tested beetles on faba bean at rates between 35.20 and 42.14%. These rates resulted in populations reduction at the end of 6 month storage period at rates between 31.24 and 36.71%. In case of wheat grains the monthly rate of increase was reduced at rates between 29.05 and 46.80%. These rates resulted in populations reduction at the end of storage period at rates between 31.51 and 47.98%. The relative faba bean damage after the storage period was reduced in the range of 32.71 to 40.99% as a result of the parasitoid release. In case of wheat grains the obtained reduction in grain weight ranged between 72.67 and 81.66.}, keywords = {Anisopteromalus calandrae,biological control,coleopteran stored products beetles,Egypt}, url = {https://eajbsa.journals.ekb.eg/article_15444.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15444_244cb986cb19de62c19a9f29a6f23d41.pdf} } @article { author = {Shairra, Souad}, title = {Parasitizm of Locust by Entomopathogenic Nematode in Relation to Insect Microaggregation Inhibitor}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {221-230}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15445}, abstract = { The present work mainly focused on the susceptibility of Schistocerca gregaria (Forskal( 5th instar nymphs to the entomopathogenic nematodes, Steinernema glaseri (Rhabditida: Steinernematidae) as a natural exposure experiment. The entomopathogenic nematode, S. glaseri turned out to be successful parasitoid of the orthopteran insect S.  gregaria. The death rate of locusts (sprayed with nematode on clover leafs) was remarkably high. Nematode killed approximately 65% of the locust with in 72 hours post infection at semi-field trial. The impact of parasitation on locusts' immune defense was closely investigated for S. gregaria parasitized by S. glaseri.  Nymphs died within 48-72h after being fed clover leafs contaminated with 1500/ml S. glaseri juveniles or previously contaminated with the dual cyclooxygenase/lipoxygenase inhibitor, phenidone. The injection of S. gregaria nymphs with, phenidone exhibited significant reduction in microaggegation in response to the nematode injections. At 12h post-injection, insignificant differences were recorded of the individual inhibitor on microaggregation, compared to the ethanol-treated (control) nymphs. Cellular defense components were strongly influenced by parasitation within the first 12h after injection of the nematodes.  Nymph's haemolymph was assayed.}, keywords = {Parasitizm,Locust,Entomopathogenic nematode}, url = {https://eajbsa.journals.ekb.eg/article_15445.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15445_f11a3b889f5d09fe13922814acb4eb42.pdf} } @article { author = {Seufi, AlaaEddeen and El Bassiony, Ghada and Ibrahim, Safinz}, title = {Purification and Characterization of Two Antimicrobial Peptides from Bacterial-challenged Haemolymph of Bombyx mori Larva.}, journal = {Egyptian Academic Journal of Biological Sciences. A, Entomology}, volume = {2}, number = {2}, pages = {231-243}, year = {2009}, publisher = {Egyptian Society of Biological Sciences}, issn = {1687-8809}, eissn = {2090-0813}, doi = {10.21608/eajbsa.2009.15446}, abstract = {Defense peptides and proteins constitute key factors in insect humoral immune response against invading microorganisms. In this study, biochemical approach was designed to purify and characterize two peptides which appeared in larval haemolymph of B. mori after bacterial challenge. The results showed a significant increase of total protein of the bacterial-challenged haemolymph and then declined over time. This suggested that the AMPs are upregulated and released in haemolymph as “acute phase response” of the insect. Full antimicrobial activity was observed for the immune haemolymph at 24 h p.i. Fractionation of the immune haemolymph extract on a reversed phase C-18 column allowed effective separation of 5 fractions containing mainly proteins and peptides of molecular masses below 20 kDa. After fractionation, one out of three fractions (fraction# 5) exhibited the strongest antimicrobial activity. Finally, two peptides (5.8 and 4.3 KDa) were purified and one of them (4.3 KDa) showed full antimicrobial activity and very weak hemolytic activity up to concentration of 100 µg/ ml. These results were consistent to the results of quantitative protein analysis. Conclusively, this study demonstrated that the antimicrobial activity of the immune haemplymph is related to the presence of two antimicrobial peptides.}, keywords = {Bombyx mori,antimicrobial peptides,HPLC,hemolytic activity}, url = {https://eajbsa.journals.ekb.eg/article_15446.html}, eprint = {https://eajbsa.journals.ekb.eg/article_15446_da6dc5c347b7df74302a793d6d41baf5.pdf} }