ORIGINAL_ARTICLE
Estimated food consumption and feeding effect with different host plants on the development and reproductive capacity of Spodoptera littoralis (boisd.) (lepidoptera: noctuidae)
Studies on effect of thirteen host plants on food consumption and somebiological aspects for larvae of the cotton leafworm, Spodoptera littoralis (Boisd.)were carried out under laboratory conditions. The results revealed that, the highestmeans of larval consumption were 0.355, 0.353 gm obtained from larva fed on tomatoleaves daily, followed by 0.34, 0.37 gm on lettuce daily, while the lowest means were0.086, 0.089 gm of radish. The highest total consumption per larva of S. littoraliswere 6.13 and 6.20 gm, when larvae fed on tomato leaves, while the lowest totalconsumption per larva were 1.43 and 1.15 gm, when larvae were fed on radish, in thetwo generations, respectively. On the other hand, larvae that fed on turnip leaves hadthe shortest larval duration 12.64 & 13.0 days, while those fed on zorbeyhh leaveshad the longest one 20.89 & 21.62 days, in the two generations, respectively. In thecase of pupae, the heaviest pupa (0.44& 0.44 gm) was obtained from larvae fed oncastor bean oil leaves. However, the lightest pupa (0.14 & 0.16g) was obtained fromlarvae fed on Radish and zorbeyhh leaves, respectively in both generations. In thecase of the moth, longevity was also affected by larval food. Males and Femalesemerging from larvae fed on tomato leaves had the longest life span 11.78 & 11.11days, respectively; while males resulting from larvae fed on zorbeyhh leaves orcabbage showed the shortest life span (5.89 & 5.95 days), but females produced fromlarvae fed on purslane or pepper leaves had the shortest life span (5.17 & 5.56 days)in the first generation. While males produced from larvae fed on pepper leaves orcabbage showed the shortest life span (6.22 & 6.42 days); purslane or cabbage leaveshad the shortest life span (5.42 & 5.56 days) in the second generation. Female mothsproduced from larvae fed on castor bean oil leaves laid the highest number of eggs2171& 2139 eggs; but zorbeyhh produced the least number, 733.3 &766.9 eggs, inthe two generations, respectively.
https://eajbsa.journals.ekb.eg/article_15159_22c67997dcc84551c50fceeef089bdc8.pdf
2011-12-01
1
8
10.21608/eajbsa.2011.15159
Spodoptera littoralis
food consumption
reproductive capacity
Al-Shannaf
H.
1
Plant Protection Research Institute, Agric. Res. Center, Dokki, Giza, Egypt
AUTHOR
ORIGINAL_ARTICLE
Combined effect of ozone mixed with carbon dioxide on the mortality of five stored-product insects
A study to determine the effect of ozone mixed with carbon dioxide on controllingstored-grain insects was conducted in the storehouse. Adults of Sitophilus oryzae (L.),Tribolium castaneum (Herbst), Rhyzopertha dominica(F.), Oryzephilussurinamensis(L.) and 3th larvae of Plodia interpunctella(Hubner) were exposed to themixture of ozone and carbon dioxide. After exposure periods of 24 h, the insects weretransferred to clean jars containing food and held at 27±2ºC and 65 ±5% R.H.Experiments were performed in different heights (30, 40, 50 and 100 cm) andnutrition materials (date, wheat and rice), in penetration tests and empty-space tests.In empty-space trials, the highest mortality was for P. interpunctella. In penetrationtests, treatment with high-pressure ozone and carbon dioxide under different heightand foodstuff may result in different rates of mortality. The mixture of ozone andcarbon dioxide in the interaction between height and diet (heigh×diet) are notsignificant for the S. oryzae, T. castaneum, R. dominicaand P. interpunctellabut for O.surinamensis is significant. The influence of ozone gas and carbon dioxide in the dateis more than rice and wheat. The mixture of ozone with carbon dioxide can be assuitable fumigant for decreasing phosphine and methyl bromide under ambientstorage conditions in penetration and empty-space fumigations.
https://eajbsa.journals.ekb.eg/article_15160_d3cd4b491369e1e4e81403c8ca09345d.pdf
2011-12-01
9
19
10.21608/eajbsa.2011.15160
fumigant
foodstuff
Phosphine
Methyl bromide
Golam
Sadeghi
rsadeghi1357@yahoo.com
1
Department of Plant Protection, Agricultural Faculty, Urmia University, Urmia- IRAN
AUTHOR
Ali
Pourmirza
2
Department of Plant Protection, Agricultural Faculty, Urmia University, Urmia- IRAN
AUTHOR
Mohammad
Safaralizade
3
Department of Plant Protection, Agricultural Faculty, Urmia University, Urmia- IRAN
AUTHOR
ORIGINAL_ARTICLE
Screening of some seaweeds species from South Sinai, Red Sea as potential bioinsecticides against mosquito larvae; Culex pipiens
On searching for bioinsecticides virulent to mosquito larvae; Culex pipiens, nineseaweeds species were screened. In this study, different dried seaweeds were appliedinto the mosquito larvae rearing jars. The effect of these seaweeds on the survival,larval development and growth rate of the mosquito Culex pipiens larvae wasmeasured. The percentage of dead larvae fed with seaweeds species after 10 and 15days were 50%, 90% respectively. Delayed pupation and body size reduction of themosquitoes larvae fed with seaweeds were observed. The study showed that Caulerpaprolifera, Caulerpa serrulata, Jania rubens, Nitophyllum punctatum, Cystoseiramyrica and Padina pavonica have potential to be used as larvicidal agents actingmainly to increase life cycle intervals and pupation delay.
https://eajbsa.journals.ekb.eg/article_15161_b0c5413ed23b73faec5e88caa40eded7.pdf
2011-12-01
21
30
10.21608/eajbsa.2011.15161
Seaweeds
chemical defense
bioinsecticides
Culex pipiens larvae
Shereen
Elbanna
sh_elbana@hotmail.com
1
Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
AUTHOR
Muhammad
. Hegazi
2
Department of Marine Biology, Faculty of Science, Suez Canal University, Ismailia, Egypt
AUTHOR
ORIGINAL_ARTICLE
Evaluation of leaves extract of Solanum melongena and Cyperus rotundus against Sitophilus Oryzae L.(Coleoptera:Curculionidae)
This study was conductedto evaluate Toxicity and repellent effects of two locally available plantsnamely Solanum melongena (Solanacae) and Cyperus rotundus (Cyperacae)against adults of Sitophilus Oryzae. The plant extracts were applied atfive concentrations on rice grains which 2.5, 5, 10, 25 and 50%) for each.. Adultinsects were exposed to treated rice grains and mortality was calculated after 1, 2, 6, 10 and 17 days of exposure. Theresults indicated that the toxic and repellent effects were proportional toconcentrations and time intervals. Higher concentrations had stronger toxicity andrepellent effects than lower concentrations. Rice grains weevils which weretreated with two plant extracts exhibited significant mortality percentage of 76.6and 46.66, respectively, after 17 days at 50% concentration. These indicatedthat S. melongena was more toxic than C. rotundus againstadult insects. The highest concentrations of 50% extracts giving 56.6 and 51.6repellency respectively in both plant extracts percentage
https://eajbsa.journals.ekb.eg/article_15162_86d54dce4ab2e73b35954130b8d0b6a0.pdf
2011-12-01
31
37
10.21608/eajbsa.2011.15162
Sitophilus oryzae
extracts
Solanum melongenae
Cyperus rotundu
mortality
repellency
Olfat
El Monairy
1
Benha University, Faculty of Science, Entomology department.
AUTHOR
Aida
Kamel
2
Benha University, Faculty of Science, Entomology department.
AUTHOR
ORIGINAL_ARTICLE
Bahram Faridi1; Ali Asghr Pourmirza1; Mohammad Hossien Safaralizadeh1 and Mohammad Vali Taghaddosi2
The food consumption pattern of Colorado potato beetle larvae revealed that the food intake increased exponentially with larval age, with the penultimate and ultimate instars larvae consuming18 and 73% of the total, respectively. Different larval stages of Colorado potato beetlewere treated by different doses of Bcillus thuringiensis Berliner var. tenebrionis under laboratory conditions to determine larval susceptibility to this bioinsecticide. The results indicated that in terms of LD50 values younger larvae were more susceptible to the B. thuringiensis than older instars. LD50 values estimated from the collected data indicated a different pattern of susceptibility of larvae to B.t. for different larval stages, as evidenced by non overlapping confidence intervals of relative median potency estimates The LT50 values showed that the speed of larval death depends on the larval age and B. thuringiensis dose. It was concluded that, if the pattern of food consumption and susceptibility in the present study was used as a model of larval food intake and susceptibility under natural conditions, the larvae must be killed prior to penultimate instar.
https://eajbsa.journals.ekb.eg/article_15163_4c8e24f3b156769779c02a54b9f653c3.pdf
2011-12-01
39
46
10.21608/eajbsa.2011.15163
feeding activity
frass production
Bioassay
lethal dose (LD50)
Bacillus thuringiensis
Bahram
Faridi
1
Department of Entomology, Faculty of Agriculture ,Urmia University, Urmia, Iran
AUTHOR
Ali
Pourmirza
2
Department of Entomology, Faculty of Agriculture ,Urmia University, Urmia, Iran
AUTHOR
Mohammad
Safaralizadeh
3
Department of Entomology, Faculty of Agriculture ,Urmia University, Urmia, Ir
AUTHOR
Mohammad
Taghaddosi
4
Department of Entomology, Agricultural and Natural Resources Research Center of Zanjan
AUTHOR
ORIGINAL_ARTICLE
The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad
The susceptibility of Leptinotarsadecemlineata (Say) adults to spinosad was determined by leaf disk and glassjar techniques under laboratory conditions. The mortalities of the treatedadults were recorded 72 h post treatments. LC50s values in leaf diskand glass jar methods for adults were 98.50 and 108.34 mg a.i/L, respectively. A significant linear relationship between LC50values of individual population across test methods was detected (r = 0.93,P < 0.05). Both bioassaytechniques were suitable for monitoring resistance to spinosad in Coloradopotato beetle adult populations. Glass jar technique, however, exhibited lessvariability in LC50 estimates and showed a higher degree ofsensitivity than the leaf disk method. Filter paper and leaf disk techniquesfor larvae were two bioassay methods used to determine spinosad susceptibilityin L. decemlineata population. Both bioassay techniques exhibited a differentlevel of susceptibility of the larvae to spinosad; however, the confidenceinterval values from filter paper method were narrow than the leaf disk assaytechnique. A significant direct relationship between LC50 values ofindividual population across test methods was observed (r = 0.96, P < 0.05). The glass jar and filter paper testingmethods are simple and sensitive test techniques for measuring susceptibilityof Colorado potato beetle adults and larvae to spinosad, respectively. Theefficacy of spinosad can be conserved for a long period of time if it isjudiciously rotated with other suitable insecticides in a spray program and thenumber of application is restricted.
https://eajbsa.journals.ekb.eg/article_15164_e9e28b6c964dbc074940e8f81805e8a2.pdf
2011-12-01
47
53
10.21608/eajbsa.2011.15164
: bioassay
Colorado potato beetle
leaf disk
paper disk
toxicity
Spinosad
Bahram
Faridi
1
Department of Entomology, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
Ali
Pourmirza
2
Department of Entomology, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
Mohammad
Safaralizadeh
3
Department of Entomology, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
Mohammad
Taghaddosi
4
Agricultural and Natural Resources Research Center of Zanjan
AUTHOR
ORIGINAL_ARTICLE
Fixed precision sequential sampling plans for leaf mines of Liriomyza sativae Blanchard (Diptera: Agromyzidae) in cucumber greenhouses
This study was conducted to develop sequential sampling plans to estimate leaf mine density by Liriomyza sativae (Blanchard) at three fixed-precision levels in cucumber greenhouse. The within- greenhouse spatial patterns of leafmines were aggregated. The slopes and intercepts of Taylor’s power law did not differ between years. A fixed-precision level sampling plan was developed using the parameters of Taylor’s power law generated from total number of leafmines in a cucumber leaf at three precision levels (D) of 0.1, 0.25 and 0.29. The resulting sampling plans were tested with sequential bootstrap simulations (n=500) using 10 independent data sets for validation. Bootstrap simulation within a wide range of densities demonstrated that actual D' values at desired D= 0.29 averaged less than or equal to 0.29 in all cases. Even at the lowest density of leaf mine (0.27 mine per leaf), the actual mean D' was 0.28 at D= 0.29. This result shows that the sampling plan developed in this study is effective and reliable for estimating the mine densities in cucumber greenhouses.
https://eajbsa.journals.ekb.eg/article_15165_881b07b05846179ec9417ae12c66ec56.pdf
2011-12-01
55
63
10.21608/eajbsa.2011.15165
Vegetable leaf miner
leaf mine
sequential sampling
spatial distribution
resampling simulation
greenhouse cucumber
Peyman
Namvar
1
Department of Plant protection, Jiroft and Kahnooj Recearch center for Agriculture
AUTHOR
Mohammed
Safaralizadeh
2
Department of Entomology, Agriculture Faculty, Urmia University
AUTHOR
Valiollah
Baniameri
3
Department of Entomology, Iranian Research Institute of Plant Protection
AUTHOR
Ali
Pourmirza
4
Department of Entomology, Agriculture Faculty, Urmia University
AUTHOR
ORIGINAL_ARTICLE
Molecular Characterization, cDNA Cloning and Phylogenetic Analysis of Cecropin Gene Isolated from Bacterial-challenged Cotton Leafworm, Spodoptera littoralis
Experiments were designed and conducted to isolate and characterize the full length cDNA of cecropin gene from the cotton leafworm, Spodoptera littoralis. In addition, sequence and phylogenetic analyses of this gene were studied. Using differential display technique, a typical cationic insect cecropin gene (SpliCec) was isolated from bacterial-challenged S. littoralis haemolymph. Five differentially displayed bands were sequenced. Based on sequence analyses of the data, specific primers for the full length of cecropin were designed and successfully amplified 388 bp amplicon. The integration of the results revealed that the 388 bp-PCR product has one open reading frame (orf) of 186 bp long, including both start codon (AUG) and stop codon (UGA). The deduced amino acid sequence of SpliCec indicated that the full length prepropeptide consists of a 22-residue signal peptide, a dipeptide prosequence and a 38-residue mature peptide. The SpliCec sequence created significant similarity with many insect cecropin sequences, especially those of M. sexta and A. convolvuli.
https://eajbsa.journals.ekb.eg/article_15166_cdf270b4d6de66b0202a368262a168f6.pdf
2011-12-01
65
75
10.21608/eajbsa.2011.15166
Spodoptera littoralis
Immune Response
DD-PCR
cecropin gene
Alaa Eddeen
. Seufi
1
Entomology Department, Faculty of Science, Cairo University, Giza, Egypt
AUTHOR