Effect of Different Host Plants on The Different Haemocyte Counts and Haemocyte Viability of Larvae of Spodoptera littoralis and Agrotis ipsilon

F., El-Khayat, E.; Dahi, Hassan F.; M., Tawfik, M.; M., El-Shewy, A.

doi:10.21608/eajbsa.2020.118768

Effect of Different Host Plants on The Different Haemocyte Counts and Haemocyte Viability of Larvae of Spodoptera littoralis and Agrotis ipsilon

Document Type : Original Article

Authors

¹ Faculty of Agriculture, Moshtohor, Benha University

² Plant Protection Research Institute, Agricult. Research Center, Dokki, Giza, Egypt

³ Department of Plant Protection, Faculty. Agriculture, Tripoli University, Libya

10.21608/eajbsa.2020.118768

Abstract

The present study aimed to investigate the effect of different six host plants namely, Cabbage, alfalfa, Jaw’s mallow, lettuce, turnip, and castor leaves on the total haemocyte count (THC), percentage of haemocyte viability, and the percentage of each type of haemocyte (differential haemocyte count, DHC) in Spodoptera littoralis and Agrotis ipsilon fourth instar larvae. There are five types of haemocytes in A. ipsilon and S. littoralis larvae; Prohaemocyte, Plasmatocyte, Granulocyte, Spherocytes, and Oenocyte. Results recorded the highest value 13.3±0.68 of Prohaemocyte at lettuce and the lowest value 9.3 for Cabbage, alfalfa and turnip. On the other hand, Prohaemocyte recorded the highest value 12.6 ± 0.9 in the case of castor feeding and 8.7 ± 0.3 for turnip for S. littoralis. Plasmatocyte recorded 45.3 ± 2.9 cells/mm³for A. ipsilon feed on turnip and 47.3 ± 1.8 cells/mm³for S. littoralis feed on lettuce as the highest value while Granulocyte recorded 33 ± 4.1 and 35 ± 0.7 for A. ipsilon feed on Jaw’s mallow, respectively. Spherocytes recoded 15.3 ± 0.9 and 12.3 ± 0.9 for A. ipsilon and S. littoralis feed on alfalfa. Finally, Oenocyte recorded 7.7 ± 1.5 and 7.3 ± 1.4 for A. ipsilon and S. littoralis feed on castor. Total haemocytic count recorded 29 ± 0.3 and 36 ± 0.75 cells/mm³ as the highest value when both A. ipsilon and S. littoralis feed on Alfalfa. Also, the haemocyte viability % recorded the highest value 91.5 ± 2.6 and 96.2 ± 0.8 % for A. ipsilon and S. littoralis feed on feed on Alfalfa. The result recorded increasing DHC level after feeding of the fourth larval instar of S. littoralis and A. ipsilon on different plant hosts such an increase in DHC gives an impression that blood cells may share in detoxifying. The most obvious activity of haemocyte is phagocytosis. In this context, the most active phagocytes are the Plasmatocyte, Plasmatocyte which was implicated in the encapsulation of necrotic tissues.

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