Comparative Modelling, Toxicological and Biochemical Studies of Imidacloprid and Thiamethoxam Insecticides on the House Fly, Musca domestica L. (Diptera: Muscidae)


1 Entomology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.

2 Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

3 Pharmaceutical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt


Neonicotinoid baits are recently replacing anticholinesterase baits for adult house fly (Musca domestica L.) control. Recently, imidacloprid (IMI) and thiamethoxam (THIA) are widely used for the control of this pest. In this study both compounds were docked to elucidate their interactions with acetylcholine binding protein (AChBP). The feeding technique was used to evaluate the toxicity of tested insecticides to adult house flies. Bioassay experiments showed that the THIA was more toxic to adult house flies than IMI with LC50 values 7.27 and 21.08 ppm; respectively. The biochemical activities of acetylcholinesterase, ATPase, cytochrome P-450 and carboxylesterase were increased by insecticides treatment, while, the total protein content was reduced.