Biochemical studies on the effect of Chitin synthesis inhibitor, (flufenoxuron) and SpliNPV on the cotton leaf worm Spodopteralittoralis Bosid (Lepidoptera:Noctuidae)

Bakr, Reda F. A.; Hafez, Jehane A.; Khamiss, Omaima A.; Zyaan, Ola H.

doi:10.21608/eajbsa.2013.13361

Biochemical studies on the effect of Chitin synthesis inhibitor, (flufenoxuron) and SpliNPV on the cotton leaf worm Spodopteralittoralis Bosid (Lepidoptera:Noctuidae)

Document Type : Original Article

Authors

¹ -Entomology Department - Faculty of Science - Ain Shams University. -Biology Department, Faculty of Science, King Khalid University, Abha, Saudi Arabia

² Entomology Department - Faculty of Science - Ain Shams University

³ Genetic Engineering and Biotechnology Institute - Menofeya University

⁴ Entomology Department - Faculty of Science - Ain Shams University.

10.21608/eajbsa.2013.13361

Abstract

In this studyflufenoxuron as insect growth regualtor and SpliNPV were used to investigate their toxicity on Spodopteralittoralis. Both 2^nd and 4^th larval instars of S. littoralis were exposed to the different concentrations of flufenoxuron and SpliNPV. The biochemical effect of IGR and SpliNPV on both 2^nd and 4^th larvae instar show changes in protein electrophoretic pattern in the current study reveals differences between the untreated samples and treated one, some proteins were missed or expressed at different stages which may be responsible for all the obtained deformation. The effect of these compounds on biochemical activities were revealed after studying electrophoretic protein (fractionated protein) using PAGE in order to determine their effects on the vital system of S. littoralis, which may indicate the formation of new proteins responsible for stimulating the immune system of the insect as a result of entering foreign objects inside the body of the insect.

Keywords