The susceptibility of Leptinotarsa decemlineata (Say) adults to spinosad was determined by leaf disk and glass jar techniques under laboratory conditions. The mortalities of the treated adults were recorded 72 h post treatments. LC50s values in leaf disk and glass jar methods for adults were 98.50 and 108.34 mg a.i/L, respectively. A significant linear relationship between LC50 values of individual population across test methods was detected (r = 0.93, P < 0.05). Both bioassay techniques were suitable for monitoring resistance to spinosad in Colorado potato beetle adult populations. Glass jar technique, however, exhibited less variability in LC50 estimates and showed a higher degree of sensitivity than the leaf disk method. Filter paper and leaf disk techniques for larvae were two bioassay methods used to determine spinosad susceptibility in L. decemlineata population. Both bioassay techniques exhibited a different level of susceptibility of the larvae to spinosad; however, the confidence interval values from filter paper method were narrow than the leaf disk assay technique. A significant direct relationship between LC50 values of individual population across test methods was observed (r = 0.96, P < 0.05). The glass jar and filter paper testing methods are simple and sensitive test techniques for measuring susceptibility of Colorado potato beetle adults and larvae to spinosad, respectively. The efficacy of spinosad can be conserved for a long period of time if it is judiciously rotated with other suitable insecticides in a spray program and the number of application is restricted.
Faridi, B., Pourmirza, A., Safaralizadeh, M., & Taghaddosi, M. (2011). The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad. Egyptian Academic Journal of Biological Sciences. A, Entomology, 4(2), 47-53. doi: 10.21608/eajbsa.2011.15164
MLA
Bahram Faridi; Ali Asghr Pourmirza; Mohammad Hossien Safaralizadeh; Mohammad Vali Taghaddosi. "The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad", Egyptian Academic Journal of Biological Sciences. A, Entomology, 4, 2, 2011, 47-53. doi: 10.21608/eajbsa.2011.15164
HARVARD
Faridi, B., Pourmirza, A., Safaralizadeh, M., Taghaddosi, M. (2011). 'The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad', Egyptian Academic Journal of Biological Sciences. A, Entomology, 4(2), pp. 47-53. doi: 10.21608/eajbsa.2011.15164
VANCOUVER
Faridi, B., Pourmirza, A., Safaralizadeh, M., Taghaddosi, M. The Susceptibility of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae) to Spinosad. Egyptian Academic Journal of Biological Sciences. A, Entomology, 2011; 4(2): 47-53. doi: 10.21608/eajbsa.2011.15164
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