Insecticidal Activity of Moringa oleifera Synthesized Silver and Zinc Nanoparticles against the House Fly, Musca domestica L.

Document Type : Original Article

Author

Biological and Geological Sciences Dept., Fac. of Education, Ain Shams Univ., Egypt.

Abstract

Green synthesized
nanoparticles have been studied as  new
and more effective biopesticides against insects of medical importance and this
for their eco-friendly aspects. In the current study Moringa oleifera
leaf extract (Mo-LE) was used to synthesis silver nanoparticles (Mo-AgNPs) and
zinc oxide nanoparticles (Mo-ZnONPs). Low concentrations of Mo-LE, Mo-AgNPs andMo-ZnONPs showed the larvicidal and pupicidal toxicity
against Musca domestica. In larvicidal bioassay, the values of LC50
were 16.50, 2.03 and 6.41 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,
respectively. In addition, the LC50 was determined against pupae,
which were129.77, 9.604 and 17.10 mg/ml for Mo-LE, Mo-AgNPs andMo-ZnONPs,
respectively. Moreover, treatment larvae with LC50's of Mo-LE, Mo-AgNPs andMo-ZnONPs
produced a significant reduction (P<0.05) in the fecundity of female
and a highly significant reduction (P<0.01) in the egg hatchability.
The total protein content and activities of nonspecific esterases, acetylcholine esterase, and glutathione S-transferase enzymes were significantly declined after
larvae had fed on LC50 Mo-AgNPs andMo-ZnONPs
treated diets as compared with the control. This study indicates that it is
feasible to use M. oleifera synthesized nanoparticles as effective candidates to develop newer and cheap
control tools for immature stages of M. domestica.

Keywords