Sequences Comparison of Cytochrome Oxidase I Gene of Certain Species of Insects in Kurdistan with Other Locations in The World


1 Department of Plant Protection, Razi University, Kermanshah, Iran

2 Department of Plant Production and Genetics, Razi University, Kermanshah, Iran

3 Department of Plant Production and Genetics, Razi University, Kermanshah, Iran.

4 Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran


In the present study, sequences of COI gene of insects belong to five orders collected from seven cities in the Kurdistan province of Iran with other locations in the word was studied. Genomic DNA was extracted using the CTAB method, Cytochrome Oxidase I gene was amplified by polymerase chain reaction (PCR) using primer pair and then the sequences were analyzed. The results of the dendrogram show that a specimen of Periplaneta americana from Barcelona, Spain was in the same clade as a specimen from Nanjing, China, but Shelfordella latralis=Blatta lateralis has made a clad with PE-B Periplaneta americana specimen collected in Kurdistan, with more distance from two former specimens of Periplaneta americana from Spain and China. Also, Periplaneta, two genera Shelfordella and Blatta have composed the same clade. Two specimens of sunn pest Eurygaster integriceps, Heteroptera, Scutelleridae which made a common clade together and made another neighbor clade with Hypseloecus sp < em>. Although, Heteroptera belonged to the Miridae family. Chrysopa pallens, Chrysopidae, Neuroptera, Acanthaclisis occitanica, Myrmeleontidae and Neuroptera both collected in Kurdistan have many genetic similarities in common and have made a clade near to clade of sunn pest but more distant from clades made by Periplaneta americana of different regions of the world. Two samples of Mediterranean flour moth, Ephestia kuehniella, that collected from Kurdistan province of Iran have made a common clade with each other, and they formed a neighbor clade with a specimen of Ephestia kuehniella, from Luebeck, Germany.