Antioxidant and Anticoagulant Activities of Red Palm Weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae) Extracts

Document Type : Original Article


Department of Zoology, Faculty of Science, Al-Azhar University, Cairo 11651, Egypt.


The study focused on analyzing the antioxidant properties of extracts from red palm weevil larvae Rhynchophorus ferrugineus, obtained using hexane, n-butanol, dichloromethane, and diethyl ether. Additionally, the anticoagulant effects of these extracts were evaluated through activated partial thromboplastin time (APTT) and prothrombin time (PT) assays. Results showed that the hexane, n-butanol, dichloromethane, and diethyl ether extracts from Rh. ferrugineus larvae exhibited antioxidant activity comparable to ascorbic acid, with IC50 values of 142.67, 122.24, 231.77 and 74.95 µg/ml, respectively, compared to 3.16 µg/ml for ascorbic acid. Also, the anticoagulant activity of Rh. ferrugineus (larvae) tested extracts using APTT assay showed 33.8, 44.3 and 50.9 sec. At 25, 50 and 75 µg/ml of hexane extract, respectively. Meanwhile, Rh. ferrugineus n-butanol extract recorded 46.9, 57.2 and 65.5 sec. At the same concentrations. Also, dichloromethane and diethyl ether recorded anticoagulant activity reached 31.2, 40.6, 48.7 and 44.3, 52.9, 62.1 sec. at 25, 50 and 75 µg/ml, respectively, vs. 59.8, 86.2 and 109.7 sec. For the Heparin sodium salt, use the same concentrations. Generally, all Rh. ferrugineus larval extracts tested proved to be antioxidant agents. The anticoagulant potential of Rh. ferrugineus tested extracts were weaker than those of heparin and needed more concentration to reach the commercial anticoagulant (Heparin sodium salt) in both assays (APTT and PT).