Molecular Barcoding of Green Bottle Fly, Lucilia sericata (Diptera: Calliphoridae ) Using COI Gene Sequences In Upper Egypt.

Elshehaby, Mohamed Ibrahim; Mahran Tony, Mahran Tony; Abd Elreheem, Abd Elbaset M. A

doi:10.21608/eajbsa.2019.56312

Molecular Barcoding of Green Bottle Fly, Lucilia sericata (Diptera: Calliphoridae ) Using COI Gene Sequences In Upper Egypt.

Document Type : Original Article

Authors

Zoology Department, Faculty of Science, Al Azhar University,Assiut, Egypt

10.21608/eajbsa.2019.56312

Abstract

The mitochondrial cytochrome oxidase I (COI) gene has been proposed as standard DNA barcoding marker for the identification of organisms. COI provides an ideal species identification marker in insects, the partial mitochondrial gene of (COI) for Egyptian specimens 6 area (L. sericata ) of genus Lucilia were amplified and sequenced successfully from Egypt in addition to data collected from the Genbank from several representative taxa and neighboring countries.The obtained sequences were analyzed and edited using Bio Edit program and aligned using Basic Local Alignment Search Tool software (BLAST). Construction of phylogenetic trees was made using the Maximum Parsimony (MP) and Maximum Likelihood (ML). SDS-PAGE traditionally is a technique employed by biochemists to detect and characterize proteins in a mixture based on their molecular size. Protein was analyzed at 14% gel concentration for wide range detection of high and low molecular weight (MW) proteins. If analytical conditions are optimized and followed by sophisticated techniques, to detect any changes in protein patterns could be happened in six Egyptian specimens of Lucilia sericata according to different areas.

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